{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T14:49:07Z","timestamp":1782485347909,"version":"3.54.5"},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In this study, novel Benzimidazole\u2010Hydrazone derivatives (\n                    <jats:bold>5A\u20135J<\/jats:bold>\n                    ) were synthesized and evaluated for their in\u00a0vitro anticancer activities against Panc\u20101, SK\u2010MEL\u201030, HeLa, and BEAS\u20102B cell lines. WST\u20101 cytotoxicity assays revealed that compound\n                    <jats:bold>5A<\/jats:bold>\n                    displayed notable potency in SK\u2010MEL\u201030 cells (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u2009=\u200923.25\u2009\u00b1\u20091.40\u2009\u03bcM), whereas compound\n                    <jats:bold>5H<\/jats:bold>\n                    exhibited significant cytotoxicity in Panc\u20101 cells (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u2009=\u200927.6\u2009\u00b1\u20091.1\u2009\u03bcM), with minimal effects on BEAS\u20102B cells (&gt;\u2009200\u2009\u03bcM), suggesting a favorable in\u00a0vitro safety profile under the tested conditions. Flow cytometric analyses demonstrated that\n                    <jats:bold>5A<\/jats:bold>\n                    and\n                    <jats:bold>5H<\/jats:bold>\n                    induced apoptosis and enhanced the apoptotic response when combined with Cisplatin, increasing apoptotic rates up to 80% in SK\u2010MEL\u201030 and 90% in Panc\u20101 cells, respectively. Cell cycle analysis revealed that compound\n                    <jats:bold>5A<\/jats:bold>\n                    induced G1 arrest in SK\u2010MEL\u201030 cells, while compound\n                    <jats:bold>5H<\/jats:bold>\n                    caused G2\/M arrest in Panc\u20101 cells. Wound healing assays further confirmed the antimigratory potential of both compounds, alone and in combination with Cisplatin. In silico ADMET profiling provided preliminary insights into the pharmacokinetic properties of compounds\n                    <jats:bold>5A<\/jats:bold>\n                    and\n                    <jats:bold>5H<\/jats:bold>\n                    , indicating generally acceptable drug\u2010likeness parameters within the limitations of computational prediction. Molecular docking analyses suggested potential interactions with the caspase\u20103 active site; however, these findings should be interpreted as supportive computational observations and do not provide direct evidence of a specific molecular mechanism underlying the observed apoptotic effects. These findings indicated that compounds\n                    <jats:bold>5A<\/jats:bold>\n                    and\n                    <jats:bold>5H<\/jats:bold>\n                    exhibited notable in\u00a0vitro anticancer activity and may serve as potential candidates for further investigation.\n                  <\/jats:p>","DOI":"10.1111\/cbdd.70349","type":"journal-article","created":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T11:50:21Z","timestamp":1782474621000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Design, Synthesis, and In\u00a0Vitro Biological Assessment of Novel Benzimidazole and Hydrazone Derivatives Against Pancreatic Cancer and Melanoma Cells"],"prefix":"10.1111","volume":"107","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0596-834X","authenticated-orcid":false,"given":"Beyza Ecem Oz","family":"Bedir","sequence":"first","affiliation":[{"name":"Department of Medical Biology, Faculty of Medicine Ankara Yildirim Beyazit University  Ankara Turkey"},{"name":"Yenimahalle Training and Research Hospital Ankara Yildirim Beyazit University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9468-4719","authenticated-orcid":false,"given":"Tuba Ozdemir","family":"Sanci","sequence":"additional","affiliation":[{"name":"Yenimahalle Training and Research Hospital Ankara Yildirim Beyazit University  Ankara Turkey"},{"name":"Department of Histology and Embryology, Faculty of Medicine Ankara Yildirim Beyazit University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9106-3848","authenticated-orcid":false,"given":"Emine","family":"Terzi","sequence":"additional","affiliation":[{"name":"Department of Medical Biology, Faculty of Medicine Ankara Yildirim Beyazit University  Ankara Turkey"},{"name":"Yenimahalle Training and Research Hospital Ankara Yildirim Beyazit University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8120-5849","authenticated-orcid":false,"given":"Elif","family":"Ercan","sequence":"additional","affiliation":[{"name":"Department of Medical Biology, Faculty of Medicine Ankara Yildirim Beyazit University  Ankara Turkey"},{"name":"Yenimahalle Training and Research Hospital Ankara Yildirim Beyazit University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nour El\u2010Huda","family":"Daoud","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy Anadolu University  Eski\u015fehir Turkey"},{"name":"Department of Pharmaceutical Chemistry, Graduate School Anadolu University  Eski\u015fehir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1879-1034","authenticated-orcid":false,"given":"Ulviye Acar","family":"\u00c7evik","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy Anadolu University  Eski\u015fehir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,26]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/cbdd.14130"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.8b01102"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986708785133013"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.762807"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389557519666190410125910"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41389\u2010023\u201000452\u20108"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21184"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rechem.2023.100925"},{"key":"e_1_2_10_10_1","first-page":"351","volume-title":"An Overview on Biological Activity of Benzimidazole Derivatives","author":"Kabi A. 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