{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T05:59:36Z","timestamp":1785218376629,"version":"3.55.0"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Infectious diseases remain one of the major global health challenges due to their rapid transmission and evolving pathogenic mechanisms. Among them,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Candida albicans<\/jats:italic>\n                    <\/jats:styled-content>\n                    has emerged as a significant opportunistic antifungal pathogen, which is responsible for biofilm\u2010associated infections. In the present study, a library of new tetrazole bearing imidazo\u2010pyridine molecules\n                    <jats:bold>(4a\u20134j)<\/jats:bold>\n                    was synthesised and characterised through spectroscopic techniques (\n                    <jats:sup>1<\/jats:sup>\n                    H,\n                    <jats:sup>13<\/jats:sup>\n                    C and MS) for structural confirmation. Their in\u00a0vitro antifungal potential was evaluated against\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">C. albicans<\/jats:styled-content>\n                      ,\n                    <\/jats:italic>\n                    where compound\n                    <jats:bold>4d<\/jats:bold>\n                    emerged as the most promising lead among all by exhibiting a MIC of 7\u2009\u03bcg\/mL and MFC of 45\u2009\u03bcg\/mL, conquering the reference fluconazole (FLC) which showed 8\u2009\u03bcg\/mL and 64\u2009\u03bcg\/mL of MIC and MFC, respectively. Compound\n                    <jats:bold>4d<\/jats:bold>\n                    also demonstrated 75% and 86% inhibition of fungal filamentation and of biofilm formation, respectively. qRT\u2010PCR analysis confirmed the significant downregulation of important virulence genes, and SEM imaging further validated biofilm prevention. Toxicity assessment through hemolytic and cytotoxic assays on HEK293 cell line indicated low toxicity across tested concentrations. Additionally, computational evaluation including molecular docking and ADMET parameters supported the predicted binding affinities and pharmacokinetic properties of the synthesised compounds.\n                  <\/jats:p>","DOI":"10.1111\/cbdd.70359","type":"journal-article","created":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T05:44:36Z","timestamp":1784267076000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploring Biofilm Inhibition Potential of Imidazo\u2010Pyridine Linked Tetrazoles Against\n                    <scp>\n                      <i>Candida albicans<\/i>\n                    <\/scp>\n                    : Synthesis, Biological Evaluation With Computational Studies"],"prefix":"10.1111","volume":"108","author":[{"family":"Madalambika","sequence":"first","affiliation":[{"name":"Department of Studies in Chemistry, Manasagangotri University of Mysore  Mysuru Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4824-1976","authenticated-orcid":false,"given":"A. 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