{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T05:59:38Z","timestamp":1785218378795,"version":"3.55.0"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:00:00Z","timestamp":1784592000000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["EMDR\/IG\/10\u20102023\u20100000879"],"award-info":[{"award-number":["EMDR\/IG\/10\u20102023\u20100000879"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The global spread of drug\u2010resistant\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    , particularly artemisinin\u2010resistant strains, underscores the urgent need for novel antimalarial agents with distinct mechanisms of action and improved therapeutic potential. In this study, we employed an integrated in silico and in\u00a0vitro strategy to identify compounds with inhibitory activity against\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    apicoplast Gyrase B (PfGyrB). High\u2010throughput virtual screening identified hit compounds with favorable predicted interactions against the target protein, which were subsequently evaluated using biochemical ATPase inhibition and parasite growth inhibition assays. UNC8153 and Fexofenadine hydrochloride demonstrated time\u2010dependent antiplasmodial activity, with lower IC\n                    <jats:sub>50<\/jats:sub>\n                    values at 96\u2009h than at 48\u2009h under prolonged exposure conditions. UNC8153 exhibited greater antiplasmodial activity (~25\u2010fold) than the reference compound novobiocin during the second intraerythrocytic cycle. Morphological analysis indicated impaired parasite development during the second intraerythrocytic cycle under prolonged exposure conditions. Importantly, UNC8153 retained inhibitory activity against the artemisinin\u2010resistant C580Y strain and exhibited minimal cytotoxicity toward HEK\u2010293 cells under the tested experimental conditions. Structural similarity analysis indicated that the identified compounds are chemically distinct from currently used antimalarial drugs, supporting their structural novelty. Collectively, these findings identify UNC8153 as a structurally distinct new antiplasmodial scaffold warranting further mechanistic, pharmacological, and in\u00a0vivo evaluation.\n                  <\/jats:p>","DOI":"10.1111\/cbdd.70364","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T06:03:28Z","timestamp":1784700208000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Discovery of Potent Antimalarial Agents Targeting\n                    <i>Plasmodium falciparum<\/i>\n                    <scp>DNA<\/scp>\n                    Gyrase B by Integrating Computational and Experimental Approaches"],"prefix":"10.1111","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3732-5063","authenticated-orcid":false,"given":"Biswajit","family":"Naik","sequence":"first","affiliation":[{"name":"Department of Biochemistry School of Life Sciences, Central University of Rajasthan  Ajmer Rajasthan India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9449-6251","authenticated-orcid":false,"given":"Welka","family":"Sahu","sequence":"additional","affiliation":[{"name":"School of Biotechnology, Kalinga Institute of Industrial Technology  Bhubaneswar Odisha India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guneswar","family":"Sethi","sequence":"additional","affiliation":[{"name":"Animal Biotechnology and Genomics Division, National Institute of Animal Science, Rural Development Administration  Jeonbuk State Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7854-1913","authenticated-orcid":false,"given":"Cherish","family":"Prashar","sequence":"additional","affiliation":[{"name":"ICMR\u2010National Institute of Malaria  New Delhi India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Gaziabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6688-7853","authenticated-orcid":false,"given":"Gajendra Mohan","family":"Baldodiya","sequence":"additional","affiliation":[{"name":"Department of Biochemistry School of Life Sciences, Central University of Rajasthan  Ajmer Rajasthan India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8825-979X","authenticated-orcid":false,"given":"Jyoti","family":"Poswal","sequence":"additional","affiliation":[{"name":"Department of Biochemistry School of Life Sciences, Central University of Rajasthan  Ajmer Rajasthan India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2292-6635","authenticated-orcid":false,"given":"Chandi C.","family":"Mandal","sequence":"additional","affiliation":[{"name":"Department of Biochemistry School of Life Sciences, Central University of Rajasthan  Ajmer Rajasthan India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4763-8373","authenticated-orcid":false,"given":"Jeong Ho","family":"Hwang","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Technology, Konkuk University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kailash C.","family":"Panday","sequence":"additional","affiliation":[{"name":"ICMR\u2010National Institute of Malaria  New Delhi India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Gaziabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8437-5250","authenticated-orcid":false,"given":"K. 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