{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T09:21:11Z","timestamp":1787822471122,"version":"build-2784847793"},"reference-count":49,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Currently, there is a lack of effective disease\u2010modifying drugs for osteoarthritis (OA), and existing treatment methods often accompany significant adverse reactions.\n                    <jats:italic>Schisandra chinensis<\/jats:italic>\n                    (SC), as a traditional Chinese medicine with anti\u2010inflammatory activity, may have therapeutic potential for OA, but its specific molecular mechanism is not yet clear. This study adopts network pharmacology strategy to explore the potential mechanism of SC intervention in OA. Screen SC active ingredients through TCMSP database and obtain OA\u2010related targets by combining OMIM, TTD, and GeneCards databases. Venn analysis shows that there are only 5 common targets between SC and OA; Further analysis revealed that PTGS2 is the only candidate target with diagnostic value (AUC\u2009=\u20090.702,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.015), while AR, ESR1, DPP4, and CHRM2 did not show clinical diagnostic efficacy. Functional enrichment analysis suggests that common targets mainly involve steroid hormone responses and extracellular matrix (ECM) related pathways, with a significant enrichment in \u201cresponse to steroid hormones\u201d (adjusted\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20091.2\u2009\u00d7\u200910\n                    <jats:sup>\u22125<\/jats:sup>\n                    ), and GSEA also shows activation of the ECM\u2010receptor interaction pathway (NES\u2009=\u20091.87,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.002). The molecular docking results indicate that the SC active ingredients MOL008957 and MOL008978 can form stable binding with PTGS2. The qRT\u2010PCR results further confirmed that the expression of PTGS2 was significantly downregulated in the OA model after SC intervention. Single cell transcriptome analysis showed that PTGS2 was mainly enriched in monocytes and gradually increased with the pseudo temporal progression; Cell communication analysis revealed that monocytes can interact with NK cells and T cells through the MIF\u2010CD74\u2009+\u2009CXCR4 and MIF\u2010CD74\u2009+\u2009CD44 signaling axes. After virtual knockout of PTGS2, downstream regulatory networks suggest that GSN may be a key target gene, mainly involving collagen containing extracellular matrix and ECM structural components. In summary, SC may exert its anti OA effect mainly by selectively inhibiting PTGS2 and maintaining the homeostasis of ECM related pathways. PTGS2 is expected to become a candidate biomarker for OA and a potential target for SC intervention in OA, but further experimental verification of the specific efficacy and mechanism of SC active ingredients is still needed.\n                  <\/jats:p>","DOI":"10.1111\/cbdd.70368","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T09:01:32Z","timestamp":1786957292000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multi\u2010Omics Integration Identifies\n                    <scp>PTGS2<\/scp>\n                    as a Candidate Diagnostic Biomarker and Precise Therapeutic Target Related to\n                    <i>Schisandra chinensis<\/i>\n                    for Osteoarthritis"],"prefix":"10.1111","volume":"108","author":[{"given":"Lingtian","family":"Min","sequence":"first","affiliation":[{"name":"Department of Orthopedics Nantong Hospital of Traditional Chinese Medicine, Nantong Hospital to Nanjing University of Chinese Medicine  Nantong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3798-8854","authenticated-orcid":false,"given":"Yikai","family":"You","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Suqian Hospital of Integrated Chinese and Western Medicine  Suqian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-9164-6001","authenticated-orcid":false,"given":"Shiqi","family":"Ren","sequence":"additional","affiliation":[{"name":"ZUMRI \u2013 Donghai County People's Hospital Joint Lab Zhuhai UM Science and Technology Research Institute  Zhuhai China"},{"name":"Donghai County People's Hospital \u2010 Jiangnan University Smart Healthcare Joint Laboratory Donghai County People's Hospital  Lianyungang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7716-4228","authenticated-orcid":false,"given":"Cheng","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Jiangsu Province (Suqian) Hospital, Suqian First Hospital Nanjing Medical University  Suqian China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,17]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-018-0276-y"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2025.105750"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2403664"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2024.102382"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-023-01120-5"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/term.2898"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/sscp.202400006"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods13040585"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-024-03222-4"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.136843"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2024.07.014"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.25802"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.adh9751"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13020\u2010025\u201001096\u2010z"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010024\u201005364\u2010z"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1017\/erm.2018.7"},{"issue":"3","key":"e_1_2_12_18_1","article-title":"Production 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