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Although AlkB homolog 3 (ALKBH3), a demethylase for 1\u2010methyladenosine (m1A) and 3\u2010methylcytidine (m3C) in DNA and RNA, has been implicated in cancer cell proliferation, its functional role in GBM remains unclear. We aimed to characterize the role of ALKBH3 in GBM and develop and evaluate a patented ALKBH3 inhibitor, HUHS199, to identify its molecular targets and therapeutic potential. We found that ALKBH3 was highly expressed in clinical GBM specimens; its knockdown inhibited GBM cell proliferation. HUHS199 inhibited GBM cell proliferation in a dose\u2010dependent manner, induced G1 phase cell cycle arrest, and increased m1A levels in RNA. Furthermore, RNA immunoprecipitation\u2013microarray analysis using an anti\u2010m1A antibody identified growth arrest and DNA\u2010damage\u2010inducible protein GADD45 alpha (GADD45A) mRNA as a target of ALKBH3\u2010mediated demethylation. These findings suggest that ALKBH3 promotes GBM cell proliferation via m1A demethylation of\n                    <jats:italic>GADD45A<\/jats:italic>\n                    mRNA. Collectively, this study highlights the therapeutic potential of ALKBH3 inhibition and presents HUHS199 as a first\u2010in\u2010class candidate for GBM treatment.\n                  <\/jats:p>","DOI":"10.1111\/cbdd.70385","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T05:45:56Z","timestamp":1788327956000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["An\n                    <scp>ALKBH3<\/scp>\n                    Inhibitor\n                    <scp>HUHS199<\/scp>\n                    Suppresses Proliferation of Glioblastoma Cells by Targeting 1\u2010Methyladenosine on\n                    <scp>GADD45A mRNA<\/scp>"],"prefix":"10.1111","volume":"108","author":[{"given":"Manami","family":"Yamada","sequence":"first","affiliation":[{"name":"Laboratory of Stem Cell Regeneration and Adaptation, Graduate School of Pharmaceutical Sciences The University of Osaka  Suita Osaka 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