{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T09:56:38Z","timestamp":1790762198854,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T00:00:00Z","timestamp":1788652800000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Mycoplasma pneumoniae<\/jats:italic>\n                    <\/jats:styled-content>\n                    (MP) is one of the main pathogens causing atypical pneumonia in children. The susceptible population is mainly children and adolescents over 5\u2009years old, and the infection rate has increased in recent years. At present, there is limited research on the pulmonary microbiota of patients with\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Mycoplasma pneumoniae<\/jats:italic>\n                    <\/jats:styled-content>\n                    pneumonia, and the characteristics of their microbiota are not yet clear. We included MPP children in stages and established two independent cohorts. Cohort I (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u2009175) performed 16S rRNA sequencing on bronchoalveolar lavage fluid (BALF) to explore microbial genus level characteristics, while Cohort II (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200941) performed metagenomic and transcriptome sequencing to explore microbial species level characteristics and predict inter group differential metabolic pathways. Finally, a murine model infected with MP was established to validate the effects of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Prevotella melaninogenica<\/jats:italic>\n                    <\/jats:styled-content>\n                    and its metabolite butyrate. Based on Multi\u2010Omic Analysis, we discovered that\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. melaninogenica<\/jats:italic>\n                    <\/jats:styled-content>\n                    was the most discriminative species enriched in the critically ill group. Functional profiling demonstrated that butanoate metabolism pathways were significantly enriched in the severe group and positively correlated with\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. melaninogenica<\/jats:italic>\n                    <\/jats:styled-content>\n                    abundance. Transcriptomic analysis revealed that\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. melaninogenica<\/jats:italic>\n                    <\/jats:styled-content>\n                    \u2010associated host genes were significantly enriched in immune regulation pathways. Animal experiments confirmed that both\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. melaninogenica<\/jats:italic>\n                    <\/jats:styled-content>\n                    and butyrate pretreatment significantly attenuated MP\u2010induced pulmonary inflammation, pathogen load, and immune cell infiltration. Respiratory microbiota dysbiosis may be associated with MPP severity.\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Prevotella melaninogenica<\/jats:italic>\n                    <\/jats:styled-content>\n                    , a potential protective commensal enriched in severe group MPP patients, may alleviate airway inflammation through its metabolite butyrate.\n                  <\/jats:p>","DOI":"10.1111\/cbdd.70397","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T04:41:42Z","timestamp":1788756102000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>Prevotella melaninogenica<\/i>\n                    Alleviate\n                    <i>Mycoplasma pneumoniae<\/i>\n                    Infection Through the Butyrate Based on Multi\u2010Omic Analysis and Experimental Validation"],"prefix":"10.1111","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4378-1471","authenticated-orcid":false,"given":"Zhengyang","family":"Xue","sequence":"first","affiliation":[{"name":"Department of Respiratory Medicine Children's Hospital Affiliated to Nanjing Medical University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiliang","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Affiliated Children's Hospital of Jiangnan University, Wuxi Children's Hospital  Wuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Affiliated Changzhou Children's Hospital of Nantong University  Changzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"DeYu","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Respiratory 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