{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T03:50:12Z","timestamp":1791949812266,"version":"4.3.4"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:00:00Z","timestamp":1791849600000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","award":["RS\u20102023\u201000280171"],"award-info":[{"award-number":["RS\u20102023\u201000280171"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Homologous recombination (HR) is an essential pathway for preserving the genomic stability of cells by repairing DNA damage, especially double\u2010strand breaks and interstrand crosslinks. RAD51 recombinase plays a central role in the HR pathway, and when DNA damage occurs, RAD51 binds to single\u2010stranded DNA at the cleavage site to form a helical nucleoprotein filament. Therefore, pharmacological inhibition of RAD51 is emerging as an attractive strategy to block the repair mechanisms of cancer cells and maximize the therapeutic efficacy of conventional radiation and chemotherapy. Several RAD51 small\u2010molecule inhibitors, including CYT\u20100851, B02, and IBR2, have demonstrated preclinical or early clinical efficacy, but no candidate has yet received regulatory approval. In this study, we identified a novel RAD51 inhibitor from a natural product library using an in silico workflow that combines machine learning models and molecular docking simulations. Among the five machine learning models evaluated, the random forest (RF) model showed the best overall predictive performance after SMOTE\u2010based class balancing and near\u2010zero variance filtering, achieving an accuracy of 0.933, sensitivity of 0.800, specificity of 1.000, Cohen's kappa of 0.842, and ROC\u2010AUC of 0.980. Using an optimized RF model, 1142 natural compounds were virtually screened, and Madecassoside was finally selected by molecular docking analysis. Biological validation showed that MDA\u2010MB\u2010231 cells increased susceptibility to cisplatin after Madecassoside treatment and also markedly inhibited the formation of RAD51 foci induced by cisplatin, strongly inhibiting DNA damage repair. These findings suggest that Madecassoside is a promising RAD51\u2010modulating lead compound that may enhance cisplatin sensitivity by impairing RAD51\u2010mediated DNA repair.<\/jats:p>","DOI":"10.1111\/cbdd.70416","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T03:41:46Z","timestamp":1791949306000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Machine Learning\u2010Guided Identification of Madecassoside as a Potential\n                    <scp>RAD51<\/scp>\n                    Modulator That Sensitizes Breast Cancer Cells to Cisplatin"],"prefix":"10.1111","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-9946-1500","authenticated-orcid":false,"given":"Minju","family":"Oh","sequence":"first","affiliation":[{"name":"College of Pharmacy Dongduk Women's University  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mi\u2010Young","family":"Jeong","sequence":"additional","affiliation":[{"name":"College of Pharmacy Dongduk Women's University  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0786-6187","authenticated-orcid":false,"given":"Jiwon","family":"Choi","sequence":"additional","affiliation":[{"name":"College of Pharmacy Dongduk Women's University  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010genet\u2010021920\u2010092410"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1010933404324"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks353"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cmdc.201600426"},{"key":"e_1_2_9_6_1","doi-asserted-by":"crossref","unstructured":"Chen T. andC.Guestrin.2016.\u201cXGBoost: A Scalable Tree Boosting System.\u201dIn: Proceedings of the 22nd ACM SIGKDD International Conference on Knowledge Discovery and Data Mining. 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