{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T12:27:43Z","timestamp":1770812863386,"version":"3.50.1"},"reference-count":52,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,6,21]],"date-time":"2013-06-21T00:00:00Z","timestamp":1371772800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2013,7]]},"abstract":"Summary<\/jats:title>Background<\/jats:title>Eosinophil activation is the key feature of upper and lower airway inflammation in aspirin\u2010exacerbated respiratory disease (AERD<\/jats:styled-content>).<\/jats:p><\/jats:sec>Objective<\/jats:title>To investigate the mechanism of eosinophil activation and identify novel inflammatory mediators using proteomics.<\/jats:p><\/jats:sec>Methods<\/jats:title>Thirty\u2010two asthmatic subjects were enrolled: 18 AERD<\/jats:styled-content> patients who showed positive responses to the lysine\u2010aspirin nasal provocation test (L\u2010ASA NPT<\/jats:styled-content>) and 14 aspirin\u2010tolerant asthma (ATA<\/jats:styled-content>) patients who showed negative responses to the L\u2010ASA NPT<\/jats:styled-content> (control group). Nasal lavage fluid (NLF<\/jats:styled-content>) was collected before (baseline), at 10, 30 and 60\u00a0min (early response), and at 3\u00a0h (late response) after the L\u2010ASA NPT<\/jats:styled-content>. Eosinophil cationic protein (ECP<\/jats:styled-content>) and cysteinyl leucotriene (CysLT<\/jats:styled-content>) levels were measured using an ImmunoCAP<\/jats:styled-content> system and ELISA<\/jats:styled-content> respectively. To identify proteins involved in AERD<\/jats:styled-content>, comparative proteomics was applied using NLF<\/jats:styled-content>s collected before and after L\u2010ASA NPT<\/jats:styled-content>s in AERD<\/jats:styled-content> patients. The clinical relevance of identified novel proteins was evaluated by ELISA<\/jats:styled-content> using NLF<\/jats:styled-content>s from the AERD<\/jats:styled-content> and ATA<\/jats:styled-content> groups.<\/jats:p><\/jats:sec>Results<\/jats:title>Eosinophil cationic protein and CysLT<\/jats:styled-content> levels both increased significantly during the early response in AERD<\/jats:styled-content>. ECP<\/jats:styled-content> levels increased until the late response in AERD<\/jats:styled-content>, while CysLT<\/jats:styled-content> levels were not significantly increased during the late response. Proteomic analysis showed up\u2010regulation of apolipoprotein A1 (ApoA1), \u03b12\u2010macroglobulin (\u03b12M) and ceruloplasmin (CP<\/jats:styled-content>), with significant increases in NLF<\/jats:styled-content> of AERD<\/jats:styled-content> patients, which was significantly higher in AERD<\/jats:styled-content> patients with chronic rhinosinusitis. Significant correlations were noted between ECP<\/jats:styled-content> and CysLT<\/jats:styled-content>, ApoA1, \u03b12M and CP<\/jats:styled-content> levels during the early response in AERD<\/jats:styled-content> patients.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Eosinophil activation occurred in early and late responses after L\u2010ASA NPT<\/jats:styled-content> in upper airway mucosa of AERD<\/jats:styled-content> patients, where ApoA1, \u03b12M and CP<\/jats:styled-content> as well as CysLT<\/jats:styled-content> may be involved in eosinophilic inflammation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.12096","type":"journal-article","created":{"date-parts":[[2013,1,28]],"date-time":"2013-01-28T05:23:41Z","timestamp":1359350621000},"page":"730-740","source":"Crossref","is-referenced-by-count":35,"title":["Eosinophil activation and novel mediators in the aspirin\u2010induced nasal response in AERD<\/scp>"],"prefix":"10.1111","volume":"43","author":[{"given":"G.\u2010S.","family":"Choi","sequence":"first","affiliation":[{"name":"Department of Internal Medicine Kosin University College of Medicine Busan South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.\u2010H.","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Pulmonary, Allergy, and Critical Care Medicine Department of Internal Medicine Hallym University Sacred Heart Hospital Anyang South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.\u2010S.","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Allergy and Clinical Immunology Ajou University School of Medicine Suwon Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.\u2010M.","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Allergy and Clinical Immunology Ajou University School of Medicine Suwon Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.\u2010H.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Allergy and Clinical Immunology Ajou University School of Medicine Suwon Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.\u2010S.","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Allergy and Clinical Immunology Ajou University School of Medicine Suwon Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,6,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-323-05659-5.00069-3"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.4168\/aair.2011.3.1.3"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2011.02557.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1081-1206(10)62084-4"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1385\/CRIAI:24:2:113"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1081-1206(10)60615-1"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2005.08.035"},{"key":"e_1_2_7_9_1","first-page":"15","article-title":"Etiology of nasal polyps associated with aspirin\u2010sensitive asthma","volume":"8","author":"Yamashita T","year":"1989","journal-title":"Rhinol Suppl"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.149.4.8143059"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-3278(03)00074-7"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.12.977"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000312669"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2006.00993.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.55.2.183"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2002.01384.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2009.02158.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.128.5.3127"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/01902140802220625"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.4168\/aair.2012.4.2.68"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00138707"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1995.tb01000.x"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.4193\/Rhino50E2"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1136\/thorax.55.11.921"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2007.01409.x"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2002.t01-1-13447.x"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2009.03216.x"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.07.034"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300562"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-006-0035-2"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2006.07.024"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2003.1487"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.3349\/ymj.2009.50.6.744"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1136\/thx.2008.101196"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1081-1206(10)62815-3"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2003.25"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1159\/000070474"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1996.tb00612.x"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2003.00276.x"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.156.5.9702072"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2008.03190.x"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0022711"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200505-740OC"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.112.300135"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M004549"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.11-0158"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)80094-1"},{"key":"e_1_2_7_48_1","doi-asserted-by":"crossref","first-page":"1535","DOI":"10.1016\/S0021-9258(19)57337-9","article-title":"Human transforming growth factor beta\u2013alpha 2\u2010macroglobulin complex is a latent form of transforming growth factor beta","volume":"263","author":"Huang SS","year":"1988","journal-title":"J Biol Chem"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2450781"},{"key":"e_1_2_7_50_1","doi-asserted-by":"crossref","first-page":"13801","DOI":"10.1016\/S0021-9258(18)80072-2","article-title":"Functional inactivation and structural disruption of human alpha 2\u2010macroglobulin by neutrophils and eosinophils","volume":"264","author":"Reddy VY","year":"1989","journal-title":"J Biol Chem"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2005.11.035"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201010-1623OC"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1155\/2000\/907478"}],"container-title":["Clinical & Experimental Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcea.12096","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cea.12096","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T23:32:59Z","timestamp":1696375979000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cea.12096"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,6,21]]},"references-count":52,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2013,7]]}},"alternative-id":["10.1111\/cea.12096"],"URL":"https:\/\/doi.org\/10.1111\/cea.12096","archive":["Portico"],"relation":{},"ISSN":["0954-7894","1365-2222"],"issn-type":[{"value":"0954-7894","type":"print"},{"value":"1365-2222","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,6,21]]}}}