{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T11:22:57Z","timestamp":1792581777030,"version":"4.5.0"},"reference-count":47,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2013,7,29]],"date-time":"2013-07-29T00:00:00Z","timestamp":1375056000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Cysteinyl leukotrienes contribute to Th2\u2010type inflammatory immune responses. Their levels in oesophageal tissue, however, do not distinguish patients with eosinophilic oesophagitis (EoE) from controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>We asked whether <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels of leukotriene C<jats:sub>4<\/jats:sub> synthase (LTC<jats:sub>4<\/jats:sub>S), a key regulator of leukotriene production, could serve as a marker for EoE.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Digital <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression profiling (nCounter<jats:sup>\u00ae<\/jats:sup> Technology) was performed on proximal and distal oesophageal biopsies of 30 paediatric EoE patients and 40 non\u2010EoE controls. Expression data were confirmed with RT\u2010<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>. LTC<jats:sub>4<\/jats:sub>S <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels were quantified in whole blood samples. Leukotriene E<jats:sub>4<\/jats:sub> was measured in urine.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>LTC<jats:sub>4<\/jats:sub>S <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels were elevated in proximal (2.6\u2010fold, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001) and distal (2.9\u2010fold, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001) oesophageal biopsies from EoE patients. Importantly, increased LTC<jats:sub>4<\/jats:sub>S <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> transcripts identified a subpopulation of EoE patients (28%). This patient subgroup had higher serum IgE levels (669\u00a0U\/mL vs. 106\u00a0U\/mL, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.01), higher <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> transcript numbers of thymic stromal lymphopoietin (TSLP) (1.6\u2010fold, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.009) and CD4 (1.4\u2010fold, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.04) but lower IL\u201023 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels (0.5\u2010fold, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.04). In contrast, elevated levels of IL\u201023 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> were found in oesophageal biopsies of patients with reflux oesophagitis. LTC<jats:sub>4<\/jats:sub>S <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> transcripts in whole blood and urinary excretion of leukotriene E<jats:sub>4<\/jats:sub> were similar in EoE patient subgroups and non\u2010EoE patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion &amp; Clinical Relevance<\/jats:title><jats:p>Elevated oesophageal expression of LTC<jats:sub>4<\/jats:sub>S <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> is found in a subgroup of EoE patients, concomitant with higher serum IgE levels and an oesophageal transcriptome indicative of a more\u2010pronounced allergic phenotype. Together with TSLP and IL\u201023 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels, oesophageal LTC<jats:sub>4<\/jats:sub>S <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> may facilitate diagnosis of an EoE subpopulation for personalized therapy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.12146","type":"journal-article","created":{"date-parts":[[2013,5,13]],"date-time":"2013-05-13T07:38:47Z","timestamp":1368430727000},"page":"902-913","source":"Crossref","is-referenced-by-count":20,"title":["Elevated levels of leukotriene C<sub>4<\/sub> synthase <scp>mRNA<\/scp> distinguish a subpopulation of eosinophilic oesophagitis patients"],"prefix":"10.1111","volume":"43","author":[{"given":"W. S.","family":"Lexmond","sequence":"first","affiliation":[{"name":"Division of Gastroenterology and Nutrition Boston Children's Hospital and Harvard Medical School Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Pardo","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Nutrition Boston Children's Hospital and Harvard Medical School Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Rooney","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Nutrition Boston Children's Hospital and Harvard Medical School Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. A.","family":"Goettel","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Nutrition Boston Children's Hospital and Harvard Medical School Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. 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