{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T08:20:48Z","timestamp":1760170848599},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,1,22]],"date-time":"2014-01-22T00:00:00Z","timestamp":1390348800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2014,2]]},"abstract":"Summary<\/jats:title>Background<\/jats:title>We recently reported that the interaction between L<\/jats:styled-content>yn and F<\/jats:styled-content>c\u03b5RI<\/jats:styled-content>\u03b2 is indispensable for F<\/jats:styled-content>c\u03b5RI<\/jats:styled-content>\u2010mediated human mast cell (MC<\/jats:styled-content>) activation and that F<\/jats:styled-content>c\u03b5RI<\/jats:styled-content>\u03b2 functions as an amplifier of F<\/jats:styled-content>c\u03b5RI<\/jats:styled-content>\u2010mediated activation signal. Some of F<\/jats:styled-content>c\u03b5RI<\/jats:styled-content>\u03b2 in cytoplasm appeared not to be co\u2010localized with F<\/jats:styled-content>c\u03b5RI<\/jats:styled-content>\u03b1. The function of F<\/jats:styled-content>c\u03b5RI<\/jats:styled-content>\u03b2 in the cytoplasm remains unknown.<\/jats:p><\/jats:sec>Methods<\/jats:title>The localization of F<\/jats:styled-content>c\u03b5RI<\/jats:styled-content>\u03b2 and F<\/jats:styled-content>c\u03b5RI<\/jats:styled-content>\u03b1 in giant papillae specimens from patients with allergic keratoconjunctivitis and of F<\/jats:styled-content>c\u03b5RI<\/jats:styled-content>\u03b2, F<\/jats:styled-content>c\u03b5RI<\/jats:styled-content>\u03b1, and L<\/jats:styled-content>yn in cultured human MC<\/jats:styled-content>s was examined using confocal microscopy. Fc\u03b5RI<\/jats:styled-content>\u03b2 was overexpressed using an adenovirus vector system. Mediators were measured by enzyme immunoassays or enzyme\u2010linked immunosorbent assays.<\/jats:p><\/jats:sec>Results<\/jats:title>In the subepithelial region, F<\/jats:styled-content>c\u03b5RI<\/jats:styled-content>\u03b2 was mainly localized in the cell membrane of MC<\/jats:styled-content>s. In the perivascular region, F<\/jats:styled-content>c\u03b5RI<\/jats:styled-content>\u03b2 expression was scattered throughout the cytoplasm and in the cell membrane of MC<\/jats:styled-content>s. Overexpression of F<\/jats:styled-content>c\u03b5RI<\/jats:styled-content>\u03b2 in MC<\/jats:styled-content>s mainly increased its cytoplasmic expression and slightly up\u2010regulated cell surface F<\/jats:styled-content>c\u03b5RI<\/jats:styled-content> expression. However, overexpression of F<\/jats:styled-content>c\u03b5RI<\/jats:styled-content>\u03b2 in MC<\/jats:styled-content>s resulted in down\u2010regulation of the tyrosine phosphorylation levels of F<\/jats:styled-content>c\u03b5RI<\/jats:styled-content>\u03b2 and S<\/jats:styled-content>yk and down\u2010regulation of the Ca2+<\/jats:sup> influx soon after Fc\u03b5RI<\/jats:styled-content> aggregation and then resulted in down\u2010regulation of degranulation, PGD<\/jats:styled-content>2<\/jats:sub> synthesis, and production of a set of cytokines. This negative regulatory effect may be due to inhibition of the redistribution of L<\/jats:styled-content>yn to small patches within the plasma membrane.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Cytoplasmic F<\/jats:styled-content>c\u03b5RI<\/jats:styled-content>\u03b2, which is not co\u2010localized with F<\/jats:styled-content>c\u03b5RI<\/jats:styled-content>\u03b1, may function as a negative regulator, as it can capture important signalling molecules such as L<\/jats:styled-content>yn.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.12210","type":"journal-article","created":{"date-parts":[[2013,10,12]],"date-time":"2013-10-12T01:10:10Z","timestamp":1381540210000},"page":"238-249","source":"Crossref","is-referenced-by-count":7,"title":["Highly expressed cytoplasmic F<\/scp>c\u03b5RI<\/scp>\u03b2 in human mast cells functions as a negative regulator of the F<\/scp>cR<\/scp>\u03b3\u2010mediated cell activation signal"],"prefix":"10.1111","volume":"44","author":[{"given":"Y.","family":"Okayama","sequence":"first","affiliation":[{"name":"Allergy and Immunology Group Research Institute of Medical Science Nihon University School of Medicine Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Matsuda","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Juntendo University School of Medicine Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.\u2010I.","family":"Kashiwakura","sequence":"additional","affiliation":[{"name":"Allergy and Immunology Group Research Institute of Medical Science Nihon University School of Medicine Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Sasaki\u2010Sakamoto","sequence":"additional","affiliation":[{"name":"Allergy and Immunology Group Research Institute of Medical Science Nihon University School of Medicine Tokyo 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