{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T20:37:43Z","timestamp":1761597463313},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,1,22]],"date-time":"2014-01-22T00:00:00Z","timestamp":1390348800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2014,2]]},"abstract":"Summary<\/jats:title>Background<\/jats:title>Defining suitable markers to diagnose and monitor allergy and its severity is essential to correctly assign patients for specific immunotherapy. Circulating levels of specific I<\/jats:styled-content>gE<\/jats:styled-content> are good markers of sensitization, but not of clinically symptomatic allergy.<\/jats:p><\/jats:sec>Objective<\/jats:title>To quantify circulating interleukin (IL<\/jats:styled-content>)\u20104\u2010 and IL<\/jats:styled-content>\u201013\u2010secreting T cells specific for house dust mite (HDM<\/jats:styled-content>) in children presenting HDM<\/jats:styled-content>\u2010allergic asthma associated or not with rhinitis and correlate results with clinical symptoms.<\/jats:p><\/jats:sec>Methods<\/jats:title>We analysed 26 children with HDM<\/jats:styled-content> respiratory disease (allergic rhinitis and asthma) together with six children with non\u2010allergic asthma. Peripheral blood mononuclear cells were stimulated with HDM<\/jats:styled-content> extract in a 24\u2010h ELIS<\/jats:styled-content>pot assay to quantify the number of HDM<\/jats:styled-content>\u2010specific IL<\/jats:styled-content>\u20104\u2010 and IL<\/jats:styled-content>\u201013\u2010secreting T cells. Asthma severity and control, and rhinitis severity were scored according to the Global Initiative for Asthma (GINA<\/jats:styled-content>) and the Allergic Rhinitis and its Impact on Asthma (ARIA<\/jats:styled-content>) Guidelines.<\/jats:p><\/jats:sec>Results<\/jats:title>The number of HDM<\/jats:styled-content>\u2010specific IL<\/jats:styled-content>\u20104\u2010 and IL<\/jats:styled-content>\u201013\u2010secreting T<\/jats:styled-content> cells was higher in patients with allergic asthma as compared to patients with non\u2010allergic asthma. It varied with the season of blood sampling with two peaks in the fall and early spring. Independently of the season, the number of HDM<\/jats:styled-content>\u2010specific IL<\/jats:styled-content>\u20104\u2010secreting T cells correlated with rhinitis severity (OR<\/jats:styled-content>\u00a0=\u00a02; 95% IC<\/jats:styled-content>:1.1\u20133.8; P<\/jats:italic>\u00a0=\u00a00.04).<\/jats:p><\/jats:sec>Conclusions and Clinical relevance<\/jats:title>Allergen\u2010specific IL<\/jats:styled-content>\u20104\u2010 and IL<\/jats:styled-content>\u201013\u2010producing T cells were only detected in HDM\u2010<\/jats:styled-content>allergic asthmatic children (not in patients with non\u2010allergic asthma). Their numbers correlated with clinical severity of allergic rhinitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.12222","type":"journal-article","created":{"date-parts":[[2013,10,21]],"date-time":"2013-10-21T14:32:21Z","timestamp":1382365941000},"page":"222-230","source":"Crossref","is-referenced-by-count":14,"title":["Quantification of circulating house dust mite\u2010specific IL<\/scp>\u20104\u2010 and IL<\/scp>\u201013\u2010secreting T<\/scp> cells correlates with rhinitis severity in asthmatic children and varies with the seasons"],"prefix":"10.1111","volume":"44","author":[{"given":"B.","family":"Michaud","sequence":"first","affiliation":[{"name":"Unit\u00e9 1013 Institut national de la sant\u00e9 et de la recherche m\u00e9dicale Paris France"},{"name":"Facult\u00e9 de M\u00e9decine Universit\u00e9 Paris Descartes Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Gouvis\u2010Echraghi","sequence":"additional","affiliation":[{"name":"Groupe hospitalier Trousseau\u2010la Roche Guyon Centre de l'Asthme et des Allergies Universit\u00e9 Pierre et Marie Curie Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Candon","sequence":"additional","affiliation":[{"name":"Unit\u00e9 1013 Institut national de la sant\u00e9 et de la recherche m\u00e9dicale Paris France"},{"name":"Facult\u00e9 de M\u00e9decine Universit\u00e9 Paris Descartes Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Couderc","sequence":"additional","affiliation":[{"name":"Groupe hospitalier Trousseau\u2010la Roche Guyon Service de Biochimie et Biologie Mol\u00e9culaire Universit\u00e9 Pierre et Marie Curie Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. P.","family":"Jais","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine Universit\u00e9 Paris Descartes Paris France"},{"name":"Biostatistique et Informatique M\u00e9dicale H\u00f4pital Necker Enfants Malades Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. F.","family":"Bach","sequence":"additional","affiliation":[{"name":"Unit\u00e9 1013 Institut national de la sant\u00e9 et de la recherche m\u00e9dicale Paris France"},{"name":"Facult\u00e9 de M\u00e9decine Universit\u00e9 Paris Descartes Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Chatenoud","sequence":"additional","affiliation":[{"name":"Unit\u00e9 1013 Institut national de la sant\u00e9 et de la recherche m\u00e9dicale Paris France"},{"name":"Facult\u00e9 de M\u00e9decine Universit\u00e9 Paris Descartes Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Just","sequence":"additional","affiliation":[{"name":"Groupe hospitalier Trousseau\u2010la Roche Guyon Centre de l'Asthme et des Allergies Universit\u00e9 Pierre et Marie Curie Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,1,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)69283-0"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00061611"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)69286-6"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2011.06.006"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2013.01.049"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2001.01161.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2001.00834.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00123411"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3038.2007.00656.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1159\/000331312"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2870"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2254"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(88)90079-8"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2009.02787.x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2011.03693.x"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(01)00535-X"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.95.08030483"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2007.01620.x"},{"key":"e_1_2_8_20_1","unstructured":"GINA \u2010 the Global Initiative for Asthma [Internet]. Disponible sur:http:\/\/www.ginasthma.org\/guidelines-gina-report-global-strategy-for-asthma.html[Last accessed 11 July 2012]"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2003.09.008"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2011-201168"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-12-95"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anai.2012.09.001"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2008.03130.x"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2007.06.034"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(00)90083-6"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(96)80008-X"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2011.02722.x"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(97)70085-X"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.6.859"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2012.07.056"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20032058"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.02.028"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.08.034"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2012.07.009"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1200850"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.06.005"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011028"},{"key":"e_1_2_8_40_1","doi-asserted-by":"crossref","first-page":"4514","DOI":"10.4049\/jimmunol.180.7.4514","article-title":"Assessment of Bet v 1\u2010specific CD4\u00a0+\u00a0 T cell responses in allergic and nonallergic individuals using MHC class II peptide tetramers","volume":"180","author":"Overtvelt L","year":"2008","journal-title":"J Immunol"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2001.056003231.x"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1994.tb00250.x"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2007.01533.x"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1995.tb01035.x"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2005.00872.x"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1081-1206(10)62993-6"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1081-1206(10)63221-8"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1997.tb01189.x"}],"container-title":["Clinical & Experimental Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcea.12222","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cea.12222","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T06:44:26Z","timestamp":1696315466000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cea.12222"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,1,22]]},"references-count":47,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,2]]}},"alternative-id":["10.1111\/cea.12222"],"URL":"https:\/\/doi.org\/10.1111\/cea.12222","archive":["Portico"],"relation":{},"ISSN":["0954-7894","1365-2222"],"issn-type":[{"value":"0954-7894","type":"print"},{"value":"1365-2222","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,1,22]]}}}