{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T03:35:01Z","timestamp":1781667301740,"version":"3.54.5"},"reference-count":28,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,4,16]],"date-time":"2014-04-16T00:00:00Z","timestamp":1397606400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Eosinophilic and non\u2010eosinophilic chronic rhinosinusitis with nasal polyps (<jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content>w<jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content>) display distinct patterns of inflammation. However, the pathogenic mechanisms underlying the heterogeneity of <jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content>w<jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content> need further investigation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate local immunoglobulin E (IgE) production and phenotype of mast cells in eosinophilic and non\u2010eosinophilic <jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content>w<jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content> in Chinese.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Total and specific IgE levels were analysed by means of the Immuno<jats:styled-content style=\"fixed-case\">CAP<\/jats:styled-content> system. The molecular steps involved in class\u2010switch recombination to IgE were investigated using <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> assays. Mast cell phenotypes, IgE\u2010 and high\u2010affinity IgE receptor (Fc\u03b5<jats:styled-content style=\"fixed-case\">RI<\/jats:styled-content>)\u2010positive cells, and allergen binding to specific IgE in sinonasal mucosa were determined by means of immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared with controls and non\u2010eosinophilic <jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content>w<jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content>, local total IgE levels were increased, and local specific IgE to common aeroallergens was more frequently found, in Chinese eosinophilic <jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content>w<jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content> independent of atopy and without significant association with <jats:italic>Staphylococcus aureus<\/jats:italic> enterotoxins. The \u03b5 germline gene transcript was also more frequently detected in eosinophilic <jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content>w<jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content>. The number of IgE\u2010 and Fc\u03b5<jats:styled-content style=\"fixed-case\">RI<\/jats:styled-content>\u2010positive cells was increased in eosinophilic <jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content>w<jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content>. Most IgE\u2010 and Fc\u03b5<jats:styled-content style=\"fixed-case\">RI<\/jats:styled-content>\u2010positive cells were mast cells. Dust mite antigens could bind to IgE on mast cells <jats:italic>in situ<\/jats:italic>. The number of mast cells positive for both tryptase and chymase and activated mast cells was increased in eosinophilic <jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content>w<jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content> and the number of activated mast cells positively correlated with local IgE level, eotaxin\u20101 level, and eosinophil count in <jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content>w<jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p>The local IgE induced by common aeroallergens may mediate mast cell activation and contribute to subsequent eosinophilic inflammation in Chinese <jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content>w<jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content>. This study offers a rationale for considering intervention strategies designed to target \u2018local allergy\u2019 in eosinophilic <jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content>w<jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.12304","type":"journal-article","created":{"date-parts":[[2014,3,6]],"date-time":"2014-03-06T07:38:43Z","timestamp":1394091523000},"page":"690-700","source":"Crossref","is-referenced-by-count":99,"title":["Increased local IgE production induced by common aeroallergens and phenotypic alteration of mast cells in Chinese eosinophilic, but not non\u2010eosinophilic, chronic rhinosinusitis with nasal polyps"],"prefix":"10.1111","volume":"44","author":[{"given":"P.\u2010P.","family":"Cao","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.\u2010N.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.\u2010F.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.\u2010H.","family":"Liu","sequence":"additional","affiliation":[{"name":"Wuhan No. 1 Hospital Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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