{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T21:39:40Z","timestamp":1790804380338,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,5,24]],"date-time":"2014-05-24T00:00:00Z","timestamp":1400889600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Allergen\u2010specific immunotherapy favours immune deviation from a Th2 to a Th1 response and increases the number of regulatory <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> cells (Tregs). Epicutaneous immunotherapy (<jats:styled-content style=\"fixed-case\">EPIT<\/jats:styled-content>) of sensitized mice decreases the clinical and the allergen\u2010specific Th2 responses and increases local and peripheral <jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>oxp3<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>regs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate the role of <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>regs in <jats:styled-content style=\"fixed-case\">EPIT<\/jats:styled-content> and characterize their phenotype and maintenance following <jats:styled-content style=\"fixed-case\">EPIT<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Tregs were investigated using <jats:italic>in vivo<\/jats:italic> depletion or adoptive transfer into <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c mice. Tregs were depleted using anti\u2010<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>25 antibody injection during <jats:styled-content style=\"fixed-case\">EPIT<\/jats:styled-content>, and allergen\u2010specific responses were compared with Sham, <jats:styled-content style=\"fixed-case\">EPIT<\/jats:styled-content> alone and na\u00efve mice. To demonstrate that Tregs can mediate protection by their own, and to study their maintenance following the end of <jats:styled-content style=\"fixed-case\">EPIT<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> CD<\/jats:styled-content>25<jats:sup>+<\/jats:sup>CD4<jats:sup>+<\/jats:sup> Tregs isolated from mice just after or 8\u00a0weeks after EPIT were transferred into peanut\u2010sensitized mice. Foxp3\u2010<jats:styled-content style=\"fixed-case\">IRES<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">mRFP<\/jats:styled-content> mice were transferred with <jats:styled-content style=\"fixed-case\">EPIT<\/jats:styled-content>\u2010induced Tregs to analyse the induction of host <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>regs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The anti\u2010<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>25 antibody injection to <jats:styled-content style=\"fixed-case\">EPIT<\/jats:styled-content> mice abrogated the induction of <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>regs in spleen and the expression of <jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>oxp3 in oesophagus. This resulted in levels of peanut\u2010induced eosinophilic infiltration in oesophagus similar to <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>ham and significantly higher than <jats:styled-content style=\"fixed-case\">EPIT<\/jats:styled-content>. Whereas the transfer of <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>regs from Sham\u2010treated mice demonstrated no effect, the transfer of <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>regs isolated just after <jats:styled-content style=\"fixed-case\">EPIT<\/jats:styled-content> prevented peanut\u2010induced eosinophil infiltration and eotaxin expression and induced <jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>oxp3 in oesophagus. The transfer of <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>regs isolated 8\u00a0weeks after <jats:styled-content style=\"fixed-case\">EPIT<\/jats:styled-content> suppressed allergen\u2010specific responses as efficiently as did Tregs isolated just after <jats:styled-content style=\"fixed-case\">EPIT<\/jats:styled-content> and increased spleen Foxp3<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>25<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> cells similarly. The use of reporter mice demonstrated an increase in host <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>regs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results confirm the <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>regs\u2010mediated mechanism of <jats:styled-content style=\"fixed-case\">EPIT<\/jats:styled-content> and demonstrate the persistence of efficient <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>regs during a long period of time after treatment cessation. This suggests that <jats:styled-content style=\"fixed-case\">EPIT<\/jats:styled-content> induces long\u2010term tolerance in peanut\u2010sensitized mice.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.12312","type":"journal-article","created":{"date-parts":[[2014,3,25]],"date-time":"2014-03-25T16:54:52Z","timestamp":1395766492000},"page":"867-881","source":"Crossref","is-referenced-by-count":73,"title":["The regulatory <scp>T<\/scp> cells induction by epicutaneous immunotherapy is sustained and mediates long\u2010term protection from eosinophilic disorders in peanut\u2010sensitized mice"],"prefix":"10.1111","volume":"44","author":[{"given":"V.","family":"Dioszeghy","sequence":"first","affiliation":[{"name":"DBV Technologies Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Mondoulet","sequence":"additional","affiliation":[{"name":"DBV Technologies Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Dhelft","sequence":"additional","affiliation":[{"name":"DBV Technologies Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Ligouis","sequence":"additional","affiliation":[{"name":"DBV Technologies Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Puteaux","sequence":"additional","affiliation":[{"name":"DBV Technologies Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Dupont","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Descartes &amp; 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