{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T20:04:59Z","timestamp":1780603499083,"version":"3.54.1"},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,1,27]],"date-time":"2015-01-27T00:00:00Z","timestamp":1422316800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Osteopontin (<jats:styled-content style=\"fixed-case\">OPN<\/jats:styled-content>) has been proposed to be associated with airway inflammation including asthma and chronic rhinosinusitis with nasal polyps (<jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content>w<jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content>). This study sought to evaluate the expression and regulation of the <jats:styled-content style=\"fixed-case\">OPN<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content>w<jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content> patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Nasal polyp (NP) tissues and normal tissues were collected from 30 CRSwNP patients and 16 control subjects. The expression and regulation of OPN, as well eosinophil (EOS) accumulation and activation, were examined in nasal tissues using DNA microarray, immunohistochemical (IHC), immunofluorescent (IF) staining and <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content> analysis. Moreover, the regulation of OPN in nasal epithelial cells and its effects on migration and activation of EOS were evaluated <jats:italic>in vitro<\/jats:italic> using flow cytometry, ELISA, <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content> and Western blot, etc.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>DNA microarray analysis identified OPN as one of the 19 upregulated genes in polyp tissues. The mean number of OPN<jats:sup>+<\/jats:sup> cells in polyp tissues was found to be significantly increased compared with the normal controls (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.01), and OPN<jats:sup>+<\/jats:sup> cells in polyp tissues significantly correlated with tissue eosinophilia [major basic protein (MBP)<jats:sup>+<\/jats:sup> cells; <jats:italic>r\u00a0<\/jats:italic>=\u00a00.51, <jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.01]. Accordingly, the <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> and protein levels of OPN in NP tissues were significantly higher than those in normal controls (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.01). Poly I:C, flagellin and TLR\u20109 agonist CpG ODN, as well as TNF\u2010\u03b1, IFN\u2010\u03b3, IL\u20106, IL\u201017A and TGF\u2010\u03b2, significantly increased OPN <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression in cultured PECs and NECs (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.05). Recombinant human OPN significantly promoted the migration of EOS, as well as enhanced EOS cationic protein (ECP) production, in an <jats:italic>in vitro<\/jats:italic> dispersed NP cells (DNPCs) culture system (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">OPN<\/jats:styled-content> promotes eosinophilic nasal inflammation in <jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content>w<jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content> patients, which may represent a promising therapeutic target.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.12320","type":"journal-article","created":{"date-parts":[[2014,4,8]],"date-time":"2014-04-08T06:56:39Z","timestamp":1396940199000},"page":"414-422","source":"Crossref","is-referenced-by-count":26,"title":["Expression and regulation of osteopontin in chronic rhinosinusitis with nasal polyps"],"prefix":"10.1111","volume":"45","author":[{"given":"W.\u2010L.","family":"Liu","sequence":"first","affiliation":[{"name":"Allergy and Cancer Center Otorhinolarygology Hospital The First Affiliated Hospital of Sun Yat\u2010sen University Guangzhou China"},{"name":"Department of Otolaryngology Guangzhou Women and Children's Medical Center Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Allergy and Cancer Center Otorhinolarygology Hospital The First Affiliated Hospital of Sun Yat\u2010sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology The Second Affiliated Hospital of Xinjiang Medical University Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.\u2010T.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology PLA General Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.\u2010H.","family":"Chen","sequence":"additional","affiliation":[{"name":"Allergy and Cancer Center Otorhinolarygology Hospital The First Affiliated Hospital of Sun Yat\u2010sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Guangzhou Women and Children's Medical Center Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Wei","sequence":"additional","affiliation":[{"name":"Allergy and Cancer Center Otorhinolarygology Hospital The First Affiliated Hospital of Sun Yat\u2010sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.\u2010Q.","family":"Sun","sequence":"additional","affiliation":[{"name":"Allergy and Cancer Center Otorhinolarygology Hospital The First Affiliated Hospital of Sun Yat\u2010sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.\u2010B.","family":"Shi","sequence":"additional","affiliation":[{"name":"Allergy and Cancer Center Otorhinolarygology Hospital The First Affiliated Hospital of Sun Yat\u2010sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.\u2010B.","family":"Li","sequence":"additional","affiliation":[{"name":"Allergy and Cancer Center Otorhinolarygology Hospital The First Affiliated Hospital of Sun Yat\u2010sen University Guangzhou 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