{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T17:13:21Z","timestamp":1788542001412,"version":"build-2803163510"},"reference-count":50,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2014,11,24]],"date-time":"2014-11-24T00:00:00Z","timestamp":1416787200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100003516","name":"Winn Feline Foundation","doi-asserted-by":"publisher","award":["MT11-002, MT12-003, MT13-002"],"award-info":[{"award-number":["MT11-002, MT12-003, MT13-002"]}],"id":[{"id":"10.13039\/100003516","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["K08AI071724"],"award-info":[{"award-number":["K08AI071724"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Frankie\u00e2\u20ac\u2122s Fund for Feline Stem Cell Research","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2014,12]]},"abstract":"Summary<\/jats:title>Background<\/jats:title>Mesenchymal stem cells (MSC<\/jats:styled-content>s) decrease airway eosinophilia, airway hyperresponsiveness (AHR<\/jats:styled-content>), and remodelling in murine models of acutely induced asthma. We hypothesized thatMSC<\/jats:styled-content>s would diminish these hallmark features in a chronic feline asthma model.<\/jats:p><\/jats:sec>Objective<\/jats:title>To document effects of allogeneic, adipose\u2010derivedMSC<\/jats:styled-content>s on airway inflammation,AHR<\/jats:styled-content>, and remodelling over time and investigate mechanisms by whichMSC<\/jats:styled-content>s alter local and systemic immunologic responses in chronic experimental feline allergic asthma.<\/jats:p><\/jats:sec>Methods<\/jats:title>Cats with chronic, experimentally induced asthma received six intravenous infusions ofMSC<\/jats:styled-content>s (0.36\u20132.5\u00a0\u00d7\u00a010E7MSC<\/jats:styled-content>s\/infusion) or placebo bimonthly at the time of study enrollment. Cats were evaluated at baseline and longitudinally for 1\u00a0year. Outcome measures included: bronchoalveolar lavage fluid cytology to assess airway eosinophilia, pulmonary mechanics and clinical scoring to assessAHR<\/jats:styled-content>, and thoracic computed tomographic (CT<\/jats:styled-content>) scans to assess structural changes (airway remodelling).CT<\/jats:styled-content>scans were evaluated using a scoring system for lung attenuation (LA<\/jats:styled-content>) and bronchial wall thickening (BWT<\/jats:styled-content>). To assess mechanisms ofMSC<\/jats:styled-content>action, immunologic assays including allergen\u2010specific IgE, cellularIL<\/jats:styled-content>\u201010 production, and allergen\u2010specific lymphocyte proliferation were performed.<\/jats:p><\/jats:sec>Results<\/jats:title>There were no differences between treatment groups or over time with respect to airway eosinophilia orAHR<\/jats:styled-content>. However, significantly lowerLA<\/jats:styled-content>andBWT<\/jats:styled-content>scores were noted inCT<\/jats:styled-content>images ofMSC<\/jats:styled-content>\u2010treated animals compared to placebo\u2010treated cats at month 8 of the study (LA<\/jats:styled-content>P<\/jats:italic>\u00a0=\u00a00.0311;BWT<\/jats:styled-content>P<\/jats:italic>\u00a0=\u00a00.0489). No differences were noted between groups in the immunologic assays.<\/jats:p><\/jats:sec>Conclusions and Clinical Relevance<\/jats:title>When administered after development of chronic allergic feline asthma,MSC<\/jats:styled-content>s failed to reduce airway inflammation andAHR<\/jats:styled-content>. However, repeated administration ofMSC<\/jats:styled-content>s at the start of study did reduce computed tomographic measures of airway remodelling by month 8, although the effect was not sustained at month 12. Further study ofMSC<\/jats:styled-content>therapy including repeatedMSC<\/jats:styled-content>administration is warranted to assess impact on remodelling in chronic asthma.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.12411","type":"journal-article","created":{"date-parts":[[2014,9,15]],"date-time":"2014-09-15T02:46:09Z","timestamp":1410749169000},"page":"1546-1557","source":"Crossref","is-referenced-by-count":77,"title":["Long\u2010term evaluation of mesenchymal stem cell therapy in a feline model of chronic allergic asthma"],"prefix":"10.1111","volume":"44","author":[{"given":"J. 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L.","family":"Webb","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Center for Immune and Regenerative Medicine Colorado State University Fort Collins CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.\u2010H.","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences College of Veterinary Medicine Washington State University Pullman WA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. R.","family":"Dodam","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Surgery College of Veterinary Medicine University of Missouri Columbia MO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. 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