{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T19:17:30Z","timestamp":1791487050054,"version":"4.3.4"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,2,25]],"date-time":"2015-02-25T00:00:00Z","timestamp":1424822400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"A*STAR's Singapore Immunology Network","award":["SIgN 10-035"],"award-info":[{"award-number":["SIgN 10-035"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2015,3]]},"abstract":"Summary<\/jats:title>\n \n Background<\/jats:title>\n \n Shellfish allergy in\n S<\/jats:styled-content>\n ingapore is highly prevalent, and shrimp allergy is the most common.\n <\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n This study aims to evaluate the clinical characteristics and immunological phenotype of shellfish allergy in this population.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Patients with self\u2010reported shellfish allergy were recruited from outpatient clinics of three large hospitals and from a population survey. Open oral food challenges (\n OFC<\/jats:styled-content>\n ) to glass prawn (\n \n L<\/jats:styled-content>\n itopenaeus vannamei\n <\/jats:italic>\n ) and tiger prawn (\n \n P<\/jats:styled-content>\n enaeus monodon\n <\/jats:italic>\n ) were carried out on all patients except for those who had a history of severe anaphylaxis. Skin prick tests (\n SPT<\/jats:styled-content>\n ) and specific\n I<\/jats:styled-content>\n g\n E<\/jats:styled-content>\n to crude and recombinant allergens were carried out to evaluate shrimp and dust mite sensitization. Immunoblots were used to assess\n I<\/jats:styled-content>\n g\n E<\/jats:styled-content>\n \u2010binding proteins.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The 104 patients recruited were categorized into shellfish allergic (\n SA<\/jats:styled-content>\n ) when\n OFC<\/jats:styled-content>\n was positive or had a history of severe anaphylaxis (\n n<\/jats:italic>\n \u00a0=\u00a039), shellfish tolerant (\n ST<\/jats:styled-content>\n ) when\n OFC<\/jats:styled-content>\n was negative (\n n<\/jats:italic>\n \u00a0=\u00a027), and house dust mite positive controls (\n HDM<\/jats:styled-content>\n +<\/jats:sup>\n ) who were\n ST<\/jats:styled-content>\n (\n n<\/jats:italic>\n \u00a0=\u00a038). Oral symptoms (87.1%) were the predominant clinical manifestation. Positive challenge doses ranged from 2 to 80\u00a0g of cooked shrimp, with 25\/52 patients reacting to either one or both shrimps challenged. The presence of specific\n I<\/jats:styled-content>\n g\n E<\/jats:styled-content>\n to shrimp either by\n SPT<\/jats:styled-content>\n and\/or Immuno\n CAP<\/jats:styled-content>\n \u00ae<\/jats:sup>\n assay provided diagnostic test sensitivity of 82% and specificity of 22.2%. The inclusion of specific\n I<\/jats:styled-content>\n g\n E<\/jats:styled-content>\n to shrimp tropomyosin and\n I<\/jats:styled-content>\n g\n E<\/jats:styled-content>\n immunoblots with shrimp extracts did not improve the diagnostic proficiency substantially.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions and Clinical Relevance<\/jats:title>\n \n This study highlights the predominance of oral symptoms in shrimp allergy in tropical\n A<\/jats:styled-content>\n sia and that a high provocation dose may be necessary to reveal shrimp allergy. Furthermore, specific\n I<\/jats:styled-content>\n g\n E<\/jats:styled-content>\n diagnostic tests and immunoblots were of limited use in this population.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/cea.12416","type":"journal-article","created":{"date-parts":[[2014,9,25]],"date-time":"2014-09-25T00:23:16Z","timestamp":1411604596000},"page":"687-697","source":"Crossref","is-referenced-by-count":74,"title":["Clinical and immunochemical profiles of food challenge proven or anaphylactic shrimp allergy in tropical\n S<\/scp>\n ingapore"],"prefix":"10.1111","volume":"45","author":[{"given":"M.","family":"Thalayasingam","sequence":"first","affiliation":[{"name":"Paediatrics Khoo Teck Puat\u2010National University Children's Medical Institute National University Health System Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I. F. A.","family":"Gerez","sequence":"additional","affiliation":[{"name":"Paediatrics Khoo Teck Puat\u2010National University Children's Medical Institute National University Health System Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. C.","family":"Yap","sequence":"additional","affiliation":[{"name":"Paediatrics Yong Loo Lin School of Medicine National University of Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. V.","family":"Llanora","sequence":"additional","affiliation":[{"name":"Paediatrics Khoo Teck Puat\u2010National University Children's Medical Institute National University Health System Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I. P.","family":"Chia","sequence":"additional","affiliation":[{"name":"Paediatrics Yong Loo Lin School of Medicine National University of Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Chua","sequence":"additional","affiliation":[{"name":"Paediatrics Yong Loo Lin School of Medicine National University of Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. J. A. O.","family":"Lee","sequence":"additional","affiliation":[{"name":"Paediatrics Yong Loo Lin School of Medicine National University of Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. D. H.","family":"Ta","sequence":"additional","affiliation":[{"name":"Paediatrics Yong Loo Lin School of Medicine National University of Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y. K.","family":"Cheng","sequence":"additional","affiliation":[{"name":"Gleneagles Medical Center Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. Y. H.","family":"Thong","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Allergy and Immunology Tan Tock Seng Hospital Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. Y.","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Allergy and Immunology Tan Tock Seng Hospital Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. P. S.","family":"Van Bever","sequence":"additional","affiliation":[{"name":"Paediatrics Khoo Teck Puat\u2010National University Children's Medical Institute National University Health System Singapore"},{"name":"Paediatrics Yong Loo Lin School of Medicine National University of Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. P.","family":"Shek","sequence":"additional","affiliation":[{"name":"Paediatrics Khoo Teck Puat\u2010National University Children's Medical Institute National University Health System Singapore"},{"name":"Paediatrics Yong Loo Lin School of Medicine National University of Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. A.","family":"Curotto de Lafaille","sequence":"additional","affiliation":[{"name":"Singapore Immunology Network A*Star Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. W.","family":"Lee","sequence":"additional","affiliation":[{"name":"Paediatrics Khoo Teck Puat\u2010National University Children's Medical Institute National University Health System Singapore"},{"name":"Paediatrics Yong Loo Lin School of Medicine National University of Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,2,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.5415\/apallergy.2013.3.1.3"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(05)80127-7"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2008.02979.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.114708"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"5354","DOI":"10.4049\/jimmunol.151.10.5354","article-title":"Identification of tropomyosin as the major shrimp allergen and characterization of its IgE\u2010binding epitopes","volume":"151","author":"Shanti KN","year":"1993","journal-title":"J Immunol"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.11.043"},{"key":"e_1_2_7_8_1","first-page":"247","article-title":"High prevalence of shellfish and house dust mite allergies in Asia\u2010Pacific: probably not just a coincidence","volume":"30","author":"Klaewsongkram J","year":"2012","journal-title":"Asian Pac J Allergy Immunol"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1159\/000102610"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.04.016"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.07.023"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.06.003"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2004.04.018"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1993.tb04698.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2745.2002.01449.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2011.03920.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3038.2007.00674.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10126-002-0082-1"},{"key":"e_1_2_7_19_1","first-page":"16","article-title":"Current immunological and molecular biological perspectives on seafood allergy: a comprehensive review","volume":"16","author":"Leung NY","year":"2012","journal-title":"Clin Rev Allergy Immunol"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2011.02663.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(88)90888-3"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.03.010"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2003.01795.x"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-011-8284-9"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2003.1611"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1159\/000065172"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2012.04035.x"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.3109\/02770903.2010.527025"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/alr.21164"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2011.03920.x"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.10.027"}],"container-title":["Clinical & Experimental Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcea.12416","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cea.12416","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T06:38:38Z","timestamp":1696315118000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cea.12416"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2,25]]},"references-count":30,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,3]]}},"alternative-id":["10.1111\/cea.12416"],"URL":"https:\/\/doi.org\/10.1111\/cea.12416","archive":["Portico"],"relation":{},"ISSN":["0954-7894","1365-2222"],"issn-type":[{"value":"0954-7894","type":"print"},{"value":"1365-2222","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,2,25]]}}}