{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T18:12:04Z","timestamp":1778695924795,"version":"3.51.4"},"reference-count":37,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2014,11,24]],"date-time":"2014-11-24T00:00:00Z","timestamp":1416787200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The lymphocyte transformation test (<jats:styled-content style=\"fixed-case\">LTT<\/jats:styled-content>) has been promoted as <jats:italic>in\u2010vitro<\/jats:italic> test for diagnosis of drug hypersensitivity. For determination of statistical <jats:styled-content style=\"fixed-case\">LTT<\/jats:styled-content> sensitivity, series of patients with clinically uniform reactions followed by complete drug hypersensitivity work\u2010up are mandatory. Assessment of <jats:styled-content style=\"fixed-case\">LTT<\/jats:styled-content> specificity requires control patients who tolerated exposure to the drug studied.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To prospectively determine the diagnostic value of the <jats:styled-content style=\"fixed-case\">LTT<\/jats:styled-content> in a clinically and diagnostically well\u2010defined series of patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Patients with exanthematous skin eruptions after ampicillin (<jats:styled-content style=\"fixed-case\">AMP<\/jats:styled-content>) intake were included in this study. After exclusion or confirmation of delayed\u2010onset allergic <jats:styled-content style=\"fixed-case\">AMP<\/jats:styled-content> hypersensitivity by skin and provocation testing, two independent <jats:styled-content style=\"fixed-case\">LTT<\/jats:styled-content>s were performed: one standard <jats:styled-content style=\"fixed-case\">LTT<\/jats:styled-content> and a modified <jats:styled-content style=\"fixed-case\">LTT<\/jats:styled-content> with additional anti\u2010<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>3\/anti\u2010<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>28 monoclonal antibody stimulation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>By testing, delayed\u2010onset allergic <jats:styled-content style=\"fixed-case\">AMP<\/jats:styled-content> hypersensitivity was diagnosed in 11 patients and definitely ruled out in 26. The standard <jats:styled-content style=\"fixed-case\">LTT<\/jats:styled-content> reached a diagnostic sensitivity of 54.5% while the modified <jats:styled-content style=\"fixed-case\">LTT<\/jats:styled-content> yielded 72.7%. However, the methodical test modification resulted in a decline of specificity from 92.3% (standard <jats:styled-content style=\"fixed-case\">LTT<\/jats:styled-content>) to 76.9%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p>In cases of <jats:styled-content style=\"fixed-case\">AMP<\/jats:styled-content>\u2010associated exanthems, the diagnostic value of the <jats:styled-content style=\"fixed-case\">LTT<\/jats:styled-content> compared with routine allergy testing is limited. When evaluating such exanthems, provocation testing remains the gold standard. Delayed reading of intradermal skin tests remains most useful to avoid positive provocation reactions.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.12437","type":"journal-article","created":{"date-parts":[[2014,10,16]],"date-time":"2014-10-16T13:04:37Z","timestamp":1413464677000},"page":"1531-1538","source":"Crossref","is-referenced-by-count":30,"title":["Aminopenicillin\u2010associated exanthem: lymphocyte transformation testing revisited"],"prefix":"10.1111","volume":"44","author":[{"given":"A.","family":"Trautmann","sequence":"first","affiliation":[{"name":"Department of Dermatology and Allergy University Hospital W\u00fcrzburg W\u00fcrzburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. 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