{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T14:31:33Z","timestamp":1784817093467,"version":"3.55.0"},"reference-count":46,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,4,16]],"date-time":"2015-04-16T00:00:00Z","timestamp":1429142400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Fondo de Investigacion Sanitario (FIS)"},{"DOI":"10.13039\/501100008530","name":"FEDER","doi-asserted-by":"crossref","award":["FIS-PI09\/01501"],"award-info":[{"award-number":["FIS-PI09\/01501"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100008530","name":"FEDER","doi-asserted-by":"crossref","award":["FIS-PI12\/00581"],"award-info":[{"award-number":["FIS-PI12\/00581"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Miguel Servet 2 Program"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Anisakiasis is a global disease caused by the consumption of raw or lightly cooked fish parasitized with third\u2010stage <jats:italic>Anisakis<\/jats:italic> larvae. <jats:italic>Anisakis simplex<\/jats:italic> allergens may cause severe allergic reactions including angio\u2010oedema, urticaria and anaphylaxis. Approximately 80% of allergic patients have allergen\u2010specific IgE against Ani s 1, and the diagnostic value of testing for antibodies to Ani s 1 has been extensively demonstrated. However, no previous studies have investigated the molecular aspects of the allergic response to Ani s 1. Knowledge of allergen\u2010specific T cell and B cell (IgE and IgG4) epitopes is important for elucidating the immunological mechanisms underlying allergic responses, and for understanding why particular proteins behave as allergens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To elucidate the main T cell\u2010 and B cell (IgE and IgG4)\u2010 binding regions of Ani s 1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>T cell epitopes were identified by peptide proliferation assays using T cell lines derived from peripheral blood mononuclear cells of 11 patients with <jats:italic>Anisakis<\/jats:italic> allergy, and IgE and IgG4 epitopes were identified by microarray immunoassay using sera from a different group of 11 patients with <jats:italic>Anisakis<\/jats:italic> allergy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Several T cell epitopes of Ani s 1 were identified, of which Ani s 1<jats:sub>145\u2013156<\/jats:sub>, Ani s 1<jats:sub>151\u2013162<\/jats:sub> and Ani s 1<jats:sub>163\u2013171<\/jats:sub> located at the C\u2010terminal end of the protein were the most relevant. IgE and IgG4 recognized largely the same peptides, including Ani s 1<jats:sub>22\u201341<\/jats:sub>, Ani s 1<jats:sub>25\u201344<\/jats:sub>, Ani s 1<jats:sub>27\u201347<\/jats:sub>, Ani s 1<jats:sub>37\u201356<\/jats:sub> and Ani s 1<jats:sub>94\u2013113<\/jats:sub>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p>This is the first report describing the T cell epitopes of an important allergen of <jats:italic>A.\u00a0simplex<\/jats:italic>, and the first B cell epitope study of this allergen in the Spanish population. This information can help to elucidate the mechanisms underlying the allergic response to Ani s 1, potentially leading to therapeutic and diagnostic advances.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.12474","type":"journal-article","created":{"date-parts":[[2014,12,11]],"date-time":"2014-12-11T15:32:43Z","timestamp":1418311963000},"page":"994-1005","source":"Crossref","is-referenced-by-count":15,"title":["Relationships between T cell and IgE\/IgG4 epitopes of the Anisakis simplex major allergen Ani s 1"],"prefix":"10.1111","volume":"45","author":[{"given":"M.","family":"Garcia Alonso","sequence":"first","affiliation":[{"name":"Hospital La Paz Institute for Health Research IdiPaz Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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