{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T19:54:44Z","timestamp":1785873284792,"version":"3.56.0"},"reference-count":68,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,12,23]],"date-time":"2015-12-23T00:00:00Z","timestamp":1450828800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["CP10\/00502"],"award-info":[{"award-number":["CP10\/00502"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["PI13\/00935"],"award-info":[{"award-number":["PI13\/00935"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Mast cells (<jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content>s) are abundant in the inflammatory infiltrate in eosinophilic oesophagitis (EoE), but decrease with disease remission. However, their phenotype, role in the pathophysiology of the disease, and modulation after effective dietary therapy are still unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To define the phenotype of oesophageal <jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content>s, their modulation through dietary therapy, and their association with clinical manifestations of EoE.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Oesophageal mucosal samples from 10 adult patients with EoE obtained before and after effective six\u2010food elimination diet (<jats:styled-content style=\"fixed-case\">SFED<\/jats:styled-content>) therapy, as well as from 10 control subjects were analysed. Eosinophil and <jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content> density were quantified. Gene expression of chemoattractants for eosinophils (<jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>11, <jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>24, and <jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>26), <jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content>s (<jats:styled-content style=\"fixed-case\">SCF<\/jats:styled-content>), and their receptors (<jats:styled-content style=\"fixed-case\">CCR<\/jats:styled-content>3 and <jats:styled-content style=\"fixed-case\">SCFR<\/jats:styled-content>, respectively) were assessed by means of <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>. Gene and protein expression of specific <jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content> proteases (<jats:styled-content style=\"fixed-case\">CPA<\/jats:styled-content>3, <jats:styled-content style=\"fixed-case\">CMA<\/jats:styled-content>, and <jats:styled-content style=\"fixed-case\">TPSB<\/jats:styled-content>2) were evaluated with <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content> and immunofluorescence. Clinical manifestations and atopic background were recorded.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content> density was significantly increased in EoE compared with controls, decreasing after dietary treatment (18.6 to 1.44 cells\/hpf, respectively; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001). The <jats:styled-content style=\"fixed-case\">MC<jats:sub>TC<\/jats:sub><\/jats:styled-content> subtype predominated in the oesophageal mucosa (90%) in both patients with EoE and controls. Gene expression of <jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content>\u2010related proteases, eotaxins, and <jats:styled-content style=\"fixed-case\">SCF<\/jats:styled-content> were up\u2010regulated in patients with EoE, but significantly decreased after therapy, regardless of atopic background. Epithelial peaks of <jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content>s and eosinophils were significantly associated (\u03c1\u00a0=\u00a00.80) in EoE and correlated with the symptom score (\u03c1\u00a0=\u00a00.78). Gene expression of <jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content> proteases and eotaxins also correlated with the symptom score (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content> and its proteases seem to play a relevant role in the pathophysiology and symptoms of EoE, which can be reversed after effective dietary treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.12504","type":"journal-article","created":{"date-parts":[[2015,2,1]],"date-time":"2015-02-01T09:18:52Z","timestamp":1422782332000},"page":"78-91","source":"Crossref","is-referenced-by-count":37,"title":["Dietary treatment modulates mast cell phenotype, density, and activity in adult eosinophilic oesophagitis"],"prefix":"10.1111","volume":"46","author":[{"given":"\u00c1.","family":"Arias","sequence":"first","affiliation":[{"name":"Research Unit Hospital General La Mancha Centro Alc\u00e1zar de San Juan Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1183-1072","authenticated-orcid":false,"given":"A. 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