{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T01:16:09Z","timestamp":1790471769940,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,11,19]],"date-time":"2015-11-19T00:00:00Z","timestamp":1447891200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100014423","name":"Environmental Restoration and Conservation Agency","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100014423","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Ministry of Health Labour and Welfare of Japan"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Distinguishing between patients with allergic bronchopulmonary aspergillosis (<jats:styled-content style=\"fixed-case\">ABPA<\/jats:styled-content>) and <jats:italic>Aspergillus fumigatus<\/jats:italic> (Af)\u2010sensitized asthmatic patients without <jats:styled-content style=\"fixed-case\">ABPA<\/jats:styled-content> is sometimes difficult owing to the IgE\u2010cross\u2010reactivity between Af and other fungal allergens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To establish the usefulness of molecular\u2010based allergy diagnostics using allergen components from Af in distinguishing <jats:styled-content style=\"fixed-case\">ABPA<\/jats:styled-content> from Af\u2010sensitized asthma without <jats:styled-content style=\"fixed-case\">ABPA<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sera from Japanese patients with <jats:styled-content style=\"fixed-case\">ABPA<\/jats:styled-content> (<jats:italic>n<\/jats:italic>\u00a0=\u00a053) and Af\u2010sensitized asthma without <jats:styled-content style=\"fixed-case\">ABPA<\/jats:styled-content> (<jats:italic>n<\/jats:italic>\u00a0=\u00a0253) were studied. The levels of IgE and IgG antibodies to allergen components from Af and IgE antibodies to different fugal allergen extracts were measured by Immuno<jats:styled-content style=\"fixed-case\">CAP<\/jats:styled-content>. Comorbid atopic dermatitis (<jats:styled-content style=\"fixed-case\">AD<\/jats:styled-content>) was taken into consideration in the sensitization profile analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Patients with <jats:styled-content style=\"fixed-case\">ABPA<\/jats:styled-content> possessed significantly higher levels of IgE antibodies to Asp f 1, and Asp f 2 than asthmatic patients without <jats:styled-content style=\"fixed-case\">ABPA<\/jats:styled-content>. The areas under the receiver operating characteristic curves for the levels of IgE to Asp f 1 and Asp f 2 as diagnostic markers of <jats:styled-content style=\"fixed-case\">ABPA<\/jats:styled-content> were 0.75 and 0.78, respectively. The presence of IgE positivity to Asp f 1 and\/or Asp f 2 resulted in increased sensitivity while losing little specificity. Comorbid <jats:styled-content style=\"fixed-case\">AD<\/jats:styled-content> was associated with higher levels of IgE to Asp f 6 (manganese superoxide dismutase from Af, a ubiquitous pan\u2010allergen in fungi) and low but positive levels of IgE to other Af\u2010components, which hampered the serological discrimination of <jats:styled-content style=\"fixed-case\">ABPA<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p>The levels of IgE to Asp f 1 and\/or Asp f 2 can effectively differentiate <jats:styled-content style=\"fixed-case\">ABPA<\/jats:styled-content> from Af\u2010sensitized asthma, suggesting that the amounts of IgE specific for these molecules are markers for genuine Af\u2010sensitization in <jats:styled-content style=\"fixed-case\">ABPA<\/jats:styled-content>. However, comorbid <jats:styled-content style=\"fixed-case\">AD<\/jats:styled-content> must be taken into consideration in the interpretation of high IgE to Asp f 6. Establishing of IgE\u2010sensitization profiles using panel of Af\u2010allergen components provides valuable information for distinguishing genuine vs. cross\u2010reactive sensitization in Af\u2010sensitized patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.12590","type":"journal-article","created":{"date-parts":[[2015,6,27]],"date-time":"2015-06-27T17:01:40Z","timestamp":1435424500000},"page":"1790-1800","source":"Crossref","is-referenced-by-count":47,"title":["Molecular\u2010based allergy diagnosis of allergic bronchopulmonary aspergillosis in Aspergillus fumigatus\u2010sensitized Japanese patients"],"prefix":"10.1111","volume":"45","author":[{"given":"H.","family":"Tanimoto","sequence":"first","affiliation":[{"name":"Clinical Research Center for Allergy and Rheumatology Sagamihara National Hospital Sagamihara Kanagawa 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