{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T01:50:00Z","timestamp":1789350600055,"version":"build-2803163510"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,12,23]],"date-time":"2015-12-23T00:00:00Z","timestamp":1450828800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science, and Technology","doi-asserted-by":"publisher","award":["S1001055"],"award-info":[{"award-number":["S1001055"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007449","name":"Takeda Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007449","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2016,1]]},"abstract":"Summary<\/jats:title>Background<\/jats:title>Diesel exhaust particles (DEP), traffic\u2010related air pollutants, are considered environmental factors that affect allergic diseases adversely. However, the exact effect of DEP<\/jats:styled-content> on allergic rhinitis (AR<\/jats:styled-content>) is unclear.<\/jats:p><\/jats:sec>Objective<\/jats:title>We thought to investigate the effect of DEP<\/jats:styled-content> on seasonal AR<\/jats:styled-content> using a mouse model.<\/jats:p><\/jats:sec>Methods<\/jats:title>Ragweed\u2010pollen\u2010sensitized mice were nasally challenged with ragweed pollen in the presence or absence of DEP<\/jats:styled-content>. The frequency of sneezing was evaluated immediately after each nasal challenge. The expression of a tight junction (TJ<\/jats:styled-content>) protein, zonula occludens\u20101 (ZO<\/jats:styled-content>\u20101), was examined by immunohistochemistry in AR<\/jats:styled-content> mice. RPMI<\/jats:styled-content> 2650 cells were used for in\u00a0vitro<\/jats:italic> examination of paracellular permeability.<\/jats:p><\/jats:sec>Results<\/jats:title>Mice challenged with ragweed pollen plus DEP<\/jats:styled-content> showed increased frequency of sneezing compared with mice challenged with pollen alone. Interestingly, intranasal DEP<\/jats:styled-content> pretreatment before ragweed pollen challenge increased ragweed\u2010pollen\u2010induced sneezing to levels comparable with the co\u2010administration group. In vitro<\/jats:italic> examination revealed that DEP<\/jats:styled-content> reduced ZO<\/jats:styled-content>\u20101 expression in RPMI<\/jats:styled-content> 2650 cells. In addition, intranasal administration of DEP<\/jats:styled-content>, but not ragweed pollen, disrupted nasal mucosal TJ<\/jats:styled-content>s in\u00a0vivo<\/jats:italic>. The effect of a single DEP<\/jats:styled-content> treatment on ragweed\u2010induced sneezing and ZO<\/jats:styled-content>\u20101 expression persisted for at least 4\u00a0days and was inversely correlated. Finally, an antioxidant substance, N\u2010acetyl\u2010L\u2010cysteine, inhibited DEP<\/jats:styled-content>\u2010mediated TJ<\/jats:styled-content> disruption and exacerbation of sneezing in AR<\/jats:styled-content>.<\/jats:p><\/jats:sec>Conclusions and Clinical Relevance<\/jats:title>DEP<\/jats:styled-content> disrupts TJ<\/jats:styled-content>s by a reactive oxygen species\u2010mediated pathway, leading to the increased permeability of nasal epithelial cells. This may result in the promotion of allergen delivery into subepithelial tissues contributing to the exacerbation of immediate allergic responses.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.12597","type":"journal-article","created":{"date-parts":[[2015,7,23]],"date-time":"2015-07-23T06:39:23Z","timestamp":1437633563000},"page":"142-152","source":"Crossref","is-referenced-by-count":75,"title":["Diesel exhaust particles exacerbate allergic rhinitis in mice by disrupting the nasal epithelial barrier"],"prefix":"10.1111","volume":"46","author":[{"given":"A.","family":"Fukuoka","sequence":"first","affiliation":[{"name":"Laboratory of Allergic Diseases Institute for Advanced Medical Sciences Hyogo College of Medicine Nishinomiya Hyogo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Matsushita","sequence":"additional","affiliation":[{"name":"Laboratory of Allergic Diseases Institute for Advanced Medical Sciences Hyogo College of Medicine Nishinomiya Hyogo 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