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Skin testing and NAPT<\/jats:styled-content> with nO<\/jats:styled-content>le e 1 were performed. Eosinophilic cationic protein (ECP) and tryptase were measured in nasal lavages before and after NAPT<\/jats:styled-content>. Serum IgE to OL<\/jats:styled-content> allergens was measured by ELISA<\/jats:styled-content>. Basophil activation tests (BAT<\/jats:styled-content>) with OL<\/jats:styled-content> and nO<\/jats:styled-content>le e 1 and dendritic cell maturation\/proliferation studies were carried out.<\/jats:p><\/jats:sec>Results<\/jats:title>All AR<\/jats:styled-content> (12\/12) and 10\/12 (83%) of LAR<\/jats:styled-content> had a +NAPT<\/jats:styled-content> to nO<\/jats:styled-content>le e 1. ECP levels in nasal lavages were significantly increased after NAPT<\/jats:styled-content> in both AR<\/jats:styled-content> and LAR<\/jats:styled-content> compared with CG<\/jats:styled-content> at 15\u00a0min (P<\/jats:italic>\u00a0<\u00a00.05). Serum IgE was positive only in AR<\/jats:styled-content>. All AR<\/jats:styled-content> had +BAT<\/jats:styled-content> responses to OL<\/jats:styled-content> and 10\/12 to nO<\/jats:styled-content>le e 1 (83%); 8\/12 LAR<\/jats:styled-content> (66.6%) had a +BAT<\/jats:styled-content> to OL<\/jats:styled-content> and 4\/12 (33%) to nO<\/jats:styled-content>le e 1, with only one subject of the CG with a +BAT<\/jats:styled-content> to both OL<\/jats:styled-content> and nO<\/jats:styled-content>le e 1 (8%). Dendritic cell proliferation to nO<\/jats:styled-content>le e 1 was increased in AR<\/jats:styled-content> compared to LAR<\/jats:styled-content> and CG<\/jats:styled-content> (P<\/jats:italic>\u00a0=\u00a00.019 and P<\/jats:italic>\u00a0=\u00a00.001, respectively).<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Both AR<\/jats:styled-content> and LAR<\/jats:styled-content> had a similar in\u00a0vivo<\/jats:italic> response to nO<\/jats:styled-content>le e 1 with release of inflammatory mediators. Specific basophil activation with OL<\/jats:styled-content> and nO<\/jats:styled-content>le e 1 was observed in LAR<\/jats:styled-content> confirming previous data obtained with dust mites.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.12600","type":"journal-article","created":{"date-parts":[[2015,7,29]],"date-time":"2015-07-29T12:21:03Z","timestamp":1438172463000},"page":"1703-1712","source":"Crossref","is-referenced-by-count":40,"title":["Immunologic responses to the major allergen of Olea europaea<\/i> in local and systemic allergic rhinitis subjects"],"prefix":"10.1111","volume":"45","author":[{"given":"P.","family":"Campo","sequence":"first","affiliation":[{"name":"U.G.C. Allergy IBIMA Regional University Hospital of M\u00e1laga UMA M\u00e1laga Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Villalba","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Faculty of Chemistry Complutense University of Madrid Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Barrionuevo","sequence":"additional","affiliation":[{"name":"U.G.C. Allergy IBIMA Regional University Hospital of M\u00e1laga UMA M\u00e1laga Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Rond\u00f3n","sequence":"additional","affiliation":[{"name":"U.G.C. Allergy IBIMA Regional University Hospital of M\u00e1laga UMA M\u00e1laga Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Salas","sequence":"additional","affiliation":[{"name":"U.G.C. Allergy IBIMA Regional University Hospital of M\u00e1laga UMA M\u00e1laga Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Galindo","sequence":"additional","affiliation":[{"name":"U.G.C. Allergy IBIMA Regional University Hospital of M\u00e1laga UMA M\u00e1laga Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. J.","family":"Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Allergy Research Laboratory IBIMA Regional University Hospital of M\u00e1laga UMA M\u00e1laga Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. C.","family":"L\u00f3pez\u2010Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Faculty of Chemistry Complutense University of Madrid Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. A.","family":"Prieto\u2010del Prado","sequence":"additional","affiliation":[{"name":"U.G.C. 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