{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T13:08:56Z","timestamp":1785330536797,"version":"3.55.0"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,12,23]],"date-time":"2015-12-23T00:00:00Z","timestamp":1450828800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003478","name":"Ministry of Health, Labour and Welfare","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003478","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The number of reported cases of allergic reactions to sesame seeds (<jats:italic>Sesamum indicum<\/jats:italic>) has increased significantly. The specific IgE tests and skin prick tests presently available for diagnosis of sesame allergy are all based on crude sesame extract and are limited by their low clinical specificity. Thus, oral food challenge (<jats:styled-content style=\"fixed-case\">OFC<\/jats:styled-content>) is still the gold standard in the diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>The aim was to identify the allergen components useful to diagnose sesame\u2010allergic children with the goal to reduce the number of <jats:styled-content style=\"fixed-case\">OFC<\/jats:styled-content>s needed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Ninety\u2010two sesame\u2010sensitized children were consecutively enrolled and diagnosed based on <jats:styled-content style=\"fixed-case\">OFC<\/jats:styled-content> or convincing history. Specific IgE to purified native 11S globulin (nSes i 11S), 7S globulin (nSes i 7S), 2S albumin (nSes i 2S), and two recombinant 2S albumins (rSes i 1 and rSes i 2) was measured by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> and\/or Immuno<jats:styled-content style=\"fixed-case\">CAP<\/jats:styled-content> (rSes i 1\/streptavidin application).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Based on area under curve (<jats:styled-content style=\"fixed-case\">AUC<\/jats:styled-content>) values from receiver operating characteristic (<jats:styled-content style=\"fixed-case\">ROC<\/jats:styled-content>) analysis, rSes i 1 was shown to have the best diagnostic performance of the allergen components in <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>. The experimental rSes i 1 Immuno<jats:styled-content style=\"fixed-case\">CAP<\/jats:styled-content> test had larger <jats:styled-content style=\"fixed-case\">AUC<\/jats:styled-content> (0.891; 95% CI, 0.826\u20130.955) compared to the commercially available sesame Immuno<jats:styled-content style=\"fixed-case\">CAP<\/jats:styled-content> (0.697; 95% CI, 0.589\u20130.805). The clinical sensitivity and specificity for the rSes i 1 Immuno<jats:styled-content style=\"fixed-case\">CAP<\/jats:styled-content> test at optimal cut\u2010off (3.96\u00a0kU<jats:sub>A<\/jats:sub>\/L) were 86.1% and 85.7%, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion and Clinical Relevance<\/jats:title><jats:p>Sensitization to Ses i 1 is strongly associated with clinical sesame allergy. Measurement of specific IgE to rSes i 1 could reduce the numbers of <jats:styled-content style=\"fixed-case\">OFC<\/jats:styled-content>s needed.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.12626","type":"journal-article","created":{"date-parts":[[2015,8,27]],"date-time":"2015-08-27T10:21:19Z","timestamp":1440670879000},"page":"163-171","source":"Crossref","is-referenced-by-count":63,"title":["Measurement of specific IgE antibodies to Ses i 1 improves the diagnosis of sesame allergy"],"prefix":"10.1111","volume":"46","author":[{"given":"N.","family":"Maruyama","sequence":"first","affiliation":[{"name":"Laboratory of Food Quality Design and Development Graduate School of Agriculture Kyoto University Uji Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Nakagawa","sequence":"additional","affiliation":[{"name":"Department of Allergy Aichi Children's Health and Medical Center Obu Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Ito","sequence":"additional","affiliation":[{"name":"Department of Allergy Aichi Children's Health and Medical Center Obu Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Cabanos","sequence":"additional","affiliation":[{"name":"Laboratory of Food Quality Design and Development Graduate School of Agriculture Kyoto University Uji Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. P.","family":"Borres","sequence":"additional","affiliation":[{"name":"Thermo Fisher Scientific Uppsala Sweden"},{"name":"Department of Maternal and Child Health Uppsala University Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Mov\u00e9rare","sequence":"additional","affiliation":[{"name":"Thermo Fisher Scientific Uppsala Sweden"},{"name":"Department of Medical Sciences Respiratory Medicine and Allergology Uppsala University Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Thermo Fisher Scientific Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Allergy Clinical Research Center for Allergology and Rheumatology Sagamihara National Hospital Sagamihara Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Ebisawa","sequence":"additional","affiliation":[{"name":"Department of Allergy Clinical Research Center for Allergology and Rheumatology Sagamihara National Hospital Sagamihara Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,12,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-012-0267-2"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3038.2006.00506.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-3038.2003.00040.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.03.029"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2002.1s3412.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.2500\/aap.2009.30.3294"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.04.012"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2013.05.038"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.04.014"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3038.2011.01197.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1939-4551-6-17"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(01)00073-1"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2002.125487"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2006.01013.x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2007.03.041"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/00130832-200406000-00017"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874091X00802010016"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2013.04.028"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0981-9428(01)01314-6"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0278-6915(03)00107-8"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.2332\/allergolint.14-RAI-0770"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12259"},{"key":"e_1_2_8_24_1","first-page":"22","article-title":"Detection of 20\u00a0kDa and 32\u00a0kDa IgE\u2010binding proteins as the major allergens in Italian sesame seed allergic patients","volume":"46","author":"Asero R","year":"2014","journal-title":"Eur Ann Allergy Clin Immunol"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3038.2012.01332.x"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1159\/000082583"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2012.02.012"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(96)80014-5"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1159\/000053642"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2010.02532.x"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.10.008"}],"container-title":["Clinical &amp; 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