{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T09:13:12Z","timestamp":1790673192404,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,2,24]],"date-time":"2016-02-24T00:00:00Z","timestamp":1456272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81100704"],"award-info":[{"award-number":["81100704"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81441029"],"award-info":[{"award-number":["81441029"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81441031"],"award-info":[{"award-number":["81441031"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7131006"],"award-info":[{"award-number":["7131006"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2016,3]]},"abstract":"Summary<\/jats:title>Background<\/jats:title>Mucociliary dysfunction is a prominent pathophysiological feature of chronic rhinosinusitis with nasal polyps (CRS<\/jats:styled-content>wNP<\/jats:styled-content>); however, the precise mechanisms underlying mucociliary dysfunction are still unclear.<\/jats:p><\/jats:sec>Objective<\/jats:title>The aim of this study was therefore to evaluate the effects of IFN<\/jats:styled-content>\u2010\u03b3, IL<\/jats:styled-content>\u201013, and IL<\/jats:styled-content>\u201017 on human nasal mucociliary differentiation and ciliary beat frequency (CBF<\/jats:styled-content>) in patients with CRS<\/jats:styled-content>wNP<\/jats:styled-content>.<\/jats:p><\/jats:sec>Methods<\/jats:title>Human nasal epithelial cells from tissue of patients with CRS<\/jats:styled-content>wNP<\/jats:styled-content> and control subjects were established as air\u2013liquid interface (ALI<\/jats:styled-content>) primary cultures. Confluent cultures were incubated with10\u00a0ng\/mL each of IFN<\/jats:styled-content>\u2010\u03b3, IL<\/jats:styled-content>\u201013, or IL<\/jats:styled-content>\u201017 for 14\u00a0days and assessed for expression of specific morphological markers and factors associated with mucociliary differentiation, the percentage of ciliated and goblet cells, and CBF<\/jats:styled-content>.<\/jats:p><\/jats:sec>Results<\/jats:title>In comparison with control subjects, percentage of ciliated cells and CBF<\/jats:styled-content> were decreased; while percentage of goblet cells, FOXJ<\/jats:styled-content>1,<\/jats:italic> and MUC<\/jats:styled-content>5AC<\/jats:styled-content><\/jats:italic> mRNA<\/jats:styled-content> expression were increased in nasal polyp\u2010derived epithelial cultures. Treatment with IFN<\/jats:styled-content>\u2010\u03b3 and IL<\/jats:styled-content>\u201013 significantly decreased the expression of \u03b2\u2010tubulin IV (specific cilia marker), ciliated cell number, and expression of FOXJ<\/jats:styled-content>1<\/jats:italic> and DNAI<\/jats:styled-content>2<\/jats:italic>, in epithelial cultures derived from both CRS<\/jats:styled-content>wNP<\/jats:styled-content> patients and control subjects. Furthermore, while both IFN<\/jats:styled-content>\u2010\u03b3 and IL<\/jats:styled-content>\u201013 treatment significantly decreased the CBF<\/jats:styled-content> of cells from both CRS<\/jats:styled-content>wNP<\/jats:styled-content> patients and control subjects, IL<\/jats:styled-content>\u201013 additionally significantly increased goblet cell number and the expression of MUC<\/jats:styled-content>5AC<\/jats:styled-content><\/jats:italic> and CLCA<\/jats:styled-content>1<\/jats:italic>, in these cultures. IL<\/jats:styled-content>\u201017 treatment did not significantly affect ciliated or goblet cell differentiation, CBF<\/jats:styled-content>, nor MUC<\/jats:styled-content>5AC<\/jats:styled-content><\/jats:italic> and CLCA<\/jats:styled-content>1<\/jats:italic> expression, but increased both MUC<\/jats:styled-content>5B <\/jats:italic>mRNA<\/jats:styled-content> and protein expression in these cultures.<\/jats:p><\/jats:sec>Conclusion and clinical relevance<\/jats:title>The demonstration that IFN<\/jats:styled-content>\u2010\u03b3 and IL<\/jats:styled-content>\u201013 both significantly reduce ciliated cell differentiation and CBF<\/jats:styled-content> in CRS<\/jats:styled-content>wNP<\/jats:styled-content> patients, and IL<\/jats:styled-content>\u201013 additionally induces significant goblet cell hyperplasia and MUC<\/jats:styled-content>5AC<\/jats:styled-content><\/jats:italic> mucin expression, as well as IL<\/jats:styled-content>\u201017 significantly increases MUC<\/jats:styled-content>5B<\/jats:italic> mucin expression, suggests that these inflammatory cytokines may be potential therapeutic targets in the management of CRS<\/jats:styled-content>wNP<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.12644","type":"journal-article","created":{"date-parts":[[2015,9,24]],"date-time":"2015-09-24T05:40:43Z","timestamp":1443073243000},"page":"449-460","source":"Crossref","is-referenced-by-count":56,"title":["Role of IFN<\/scp>\u2010\u03b3, IL<\/scp>\u201013, and IL<\/scp>\u201017 on mucociliary differentiation of nasal epithelial cells in chronic rhinosinusitis with nasal polyps"],"prefix":"10.1111","volume":"46","author":[{"given":"J.","family":"Jiao","sequence":"first","affiliation":[{"name":"Department of Otolaryngology, Head and Neck Surgery Beijing TongRen Hospital Capital Medical University Beijing China"},{"name":"Beijing Key Laboratory of Nasal Diseases Beijing Institute of Otolaryngology Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Duan","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Nasal Diseases Beijing Institute of Otolaryngology Beijing China"},{"name":"Department of Allergy Beijing TongRen Hospital Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Head and Neck Surgery Beijing TongRen Hospital Capital Medical University Beijing China"},{"name":"Beijing Key Laboratory of Nasal Diseases Beijing Institute of Otolaryngology Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Head and Neck Surgery Beijing TongRen Hospital Capital Medical University Beijing China"},{"name":"Beijing Key Laboratory of Nasal Diseases Beijing Institute of Otolaryngology Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Head and Neck Surgery Beijing TongRen Hospital Capital Medical University Beijing China"},{"name":"Beijing Key Laboratory of Nasal Diseases Beijing Institute of Otolaryngology Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Head and Neck Surgery Beijing TongRen Hospital Capital Medical University Beijing China"},{"name":"Beijing Key Laboratory of Nasal Diseases Beijing Institute of Otolaryngology Beijing China"},{"name":"Department of Allergy Beijing TongRen Hospital Capital Medical University Beijing 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