{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T00:03:44Z","timestamp":1775606624255,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,5,3]],"date-time":"2016-05-03T00:00:00Z","timestamp":1462233600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003417","name":"Deutsche Gesetzliche Unfallversicherung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003417","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Diagnosis of mould allergy is complicated due to the heterogeneity of the test material and the decrease in the number of commercial mould skin test solutions that are currently available.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The aim of this study was to compare skin prick tests (<jats:styled-content style=\"fixed-case\">SPT<\/jats:styled-content>) from different manufacturers to one another and concurrently with <jats:styled-content style=\"fixed-case\">sIgE<\/jats:styled-content> tests for <jats:italic>Aspergillus fumigatus<\/jats:italic> (Asp f)<jats:italic>, Cladosporium herbarum<\/jats:italic> (Cla h)<jats:italic>, Penicillium chrysogenum<\/jats:italic> (Pen ch)<jats:italic>, Alternaria alternata<\/jats:italic> (Alt a) and <jats:italic>Aspergillus versicolor<\/jats:italic> (Asp v) to ascertain a feasible diagnostic procedure for mould sensitization.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this multi\u2010centre study, 168 patients with mould exposure and\/or mould\u2010induced respiratory symptoms were included. Mould <jats:styled-content style=\"fixed-case\">SPT<\/jats:styled-content> solutions were analysed biochemically and tested in duplicate on patients\u2019 arms. Specific IgE (<jats:styled-content style=\"fixed-case\">sIgE<\/jats:styled-content>) concentrations to corresponding mould species and mould mix (mx1) were measured by Immuno<jats:styled-content style=\"fixed-case\">CAP<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">SPT<\/jats:styled-content>s in accordance with one another and with <jats:styled-content style=\"fixed-case\">sIgE<\/jats:styled-content> were further considered. The test efficiency was calculated using receiver\u2010operating characteristic (<jats:styled-content style=\"fixed-case\">ROC<\/jats:styled-content>) analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Mould sensitization was more frequently detected by the <jats:styled-content style=\"fixed-case\">SPT<\/jats:styled-content> (90 of 168) than by the <jats:styled-content style=\"fixed-case\">sIgE<\/jats:styled-content> tests (56 of 168). Concordances of double <jats:styled-content style=\"fixed-case\">SPT<\/jats:styled-content> positives were only sufficient (\u2265 80%) for environmental allergens, two Asp f and three Alt a <jats:styled-content style=\"fixed-case\">SPT<\/jats:styled-content> solutions, whereas all other mould solutions revealed concordances &lt; 80%. The antigen content of <jats:styled-content style=\"fixed-case\">SPT<\/jats:styled-content> solutions was positively associated with concordant <jats:styled-content style=\"fixed-case\">SPT<\/jats:styled-content> double values as well as with <jats:styled-content style=\"fixed-case\">sIgE<\/jats:styled-content>. Taking <jats:styled-content style=\"fixed-case\">sIgE<\/jats:styled-content> as the \u2018positive standard\u2019, all mould <jats:styled-content style=\"fixed-case\">SPT<\/jats:styled-content> solutions revealed test efficiencies &gt; 80%, but varied up to 20% in sensitivity and positive predictive value with the exception of Alt a.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">SPT<\/jats:styled-content> solutions are sensitive and essential diagnostic tools for the detection of mould sensitization. Our recommendation for diagnosis would be to test at least Alt a, Asp f and Pen ch using <jats:styled-content style=\"fixed-case\">SPT<\/jats:styled-content> and additional <jats:styled-content style=\"fixed-case\">sIgE<\/jats:styled-content> test to mx1.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.12733","type":"journal-article","created":{"date-parts":[[2016,3,30]],"date-time":"2016-03-30T23:09:43Z","timestamp":1459379383000},"page":"981-991","source":"Crossref","is-referenced-by-count":22,"title":["How to diagnose mould allergy? Comparison of skin prick tests with specific IgE results"],"prefix":"10.1111","volume":"46","author":[{"given":"S.","family":"Kespohl","sequence":"first","affiliation":[{"name":"Institute for Prevention and Occupational Medicine of the German Social Accident Insurance Institute of the Ruhr\u2010Universit\u00e4t Bochum (IPA) Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Maryska","sequence":"additional","affiliation":[{"name":"Institute for Prevention and Occupational Medicine of the German Social Accident Insurance Institute of the Ruhr\u2010Universit\u00e4t Bochum (IPA) Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"B\u00fcnger","sequence":"additional","affiliation":[{"name":"Institute for Prevention and Occupational Medicine of the German Social Accident Insurance Institute of the Ruhr\u2010Universit\u00e4t Bochum (IPA) Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O.","family":"Hagemeyer","sequence":"additional","affiliation":[{"name":"Institute for Prevention and Occupational Medicine of the German Social Accident Insurance Institute of the Ruhr\u2010Universit\u00e4t Bochum (IPA) Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Jakob","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergology University Medical Center (UKGM) Justus\u2010Liebig University Giessen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Joest","sequence":"additional","affiliation":[{"name":"Malteser Lung and Allergy Centre Bonn Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Knecht","sequence":"additional","affiliation":[{"name":"Medical Practice for Pediatrics Bretten Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Koschel","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Diseases Fachkrankenhaus Coswig Centre for Pulmonary Diseases and Thoracic Surgery Coswig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Kotschy\u2010Lang","sequence":"additional","affiliation":[{"name":"Berufsgenossenschaftliche Klinik f\u00fcr Berufskrankheiten Falkenstein Falkenstein Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Merget","sequence":"additional","affiliation":[{"name":"Institute for Prevention and Occupational Medicine of the German Social Accident Insurance Institute of the Ruhr\u2010Universit\u00e4t Bochum (IPA) Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. 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