{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T19:59:51Z","timestamp":1788811191503,"version":"build-2803163510"},"reference-count":48,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2016,5,7]],"date-time":"2016-05-07T00:00:00Z","timestamp":1462579200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2016,8]]},"abstract":"Summary<\/jats:title>Background<\/jats:title>Nonsteroidal anti\u2010inflammatory drugs (NSAID<\/jats:styled-content>s) act as cofactors worsening the allergic reactions induced by food allergens.<\/jats:p><\/jats:sec>Aim<\/jats:title>The aim of this study was to evaluate the effect of both lysine acetylsalicylate (L\u2010ASA<\/jats:styled-content>) (non\u2010selective cyclooxygenase (COX<\/jats:styled-content>) inhibitor) and valdecoxib (selective COX<\/jats:styled-content>\u20102 inhibitor) in basophils activated by peach lipid transfer protein (Pru p 3) in patients with food\u2010dependent NSAID<\/jats:styled-content>\u2010induced anaphylaxis (FDNIA<\/jats:styled-content>).<\/jats:p><\/jats:sec>Methods<\/jats:title>Twenty Pru p 3\u2010allergic patients with FDNIA<\/jats:styled-content> group, eleven peach anaphylaxis not exacerbated by NSAID<\/jats:styled-content>s (no\u2010NSAID<\/jats:styled-content> group) and 5 healthy volunteers were recruited. Basophil activation (BA<\/jats:styled-content>) was measured as expression of CD<\/jats:styled-content>63 (Flow2<\/jats:sup>CAST<\/jats:styled-content>\u2122<\/jats:sup>; B\u00fchlmann\u00ae<\/jats:sup>), after stimulation with Pru p 3, both alone and in combination with L\u2010ASA<\/jats:styled-content> (1.13, 3.38 and 6.78 mm<\/jats:sc>) or valdecoxib (0.87, 7.8 and 31.25 \u03bcm<\/jats:sc>).<\/jats:p><\/jats:sec>Results<\/jats:title>Basophils from no\u2010NSAID<\/jats:styled-content> group were significantly more reactive and sensitive to Pru p 3 than those from the FDNIA<\/jats:styled-content> group. In both groups, an increase in BA<\/jats:styled-content> was observed when basophils were exposed to Pru p 3 and L\u2010ASA<\/jats:styled-content>. In the FDNIA<\/jats:styled-content> group, valdecoxib partially terminates the BA<\/jats:styled-content> induced by Pru p 3, whereas in the no\u2010NSAID<\/jats:styled-content> group, a dual effect was observed depending on the concentration tested.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>This study indicates that subjects with food\u2010induced anaphylaxis differ from FDNIA<\/jats:styled-content> subjects in the higher reactivity and sensitivity of their basophils to allergen challenge. We have shown a direct effect of NSAID<\/jats:styled-content>s on basophils using a human model of FDNIA<\/jats:styled-content>. Our results also suggest that selective COX<\/jats:styled-content>2 inhibitors might be a safe alternative. BA<\/jats:styled-content> test may be a useful tool in the study of the pathogenic mechanism of the cofactor phenomenon.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.12735","type":"journal-article","created":{"date-parts":[[2016,3,28]],"date-time":"2016-03-28T08:32:44Z","timestamp":1459153964000},"page":"1111-1119","source":"Crossref","is-referenced-by-count":36,"title":["Nonsteroidal anti\u2010inflammatory drugs enhance IgE\u2010mediated activation of human basophils in patients with food anaphylaxis dependent on and independent of nonsteroidal anti\u2010inflammatory drugs"],"prefix":"10.1111","volume":"46","author":[{"given":"M.","family":"Pascal","sequence":"first","affiliation":[{"name":"Servei d'Immunologia Centre de Diagnostic Biomedic (CDB) Hospital Clinic Universitat de Barcelona Barcelona Spain"},{"name":"Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS) Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Mu\u00f1oz\u2010Cano","sequence":"additional","affiliation":[{"name":"Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS) Barcelona Spain"},{"name":"Unitat d'Al\u00b7lergia Servei de Pneumologia Hospital Clinic Universitat de Barcelona Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Mil\u00e0","sequence":"additional","affiliation":[{"name":"Servei d'Immunologia Centre de Diagnostic Biomedic (CDB) Hospital Clinic Universitat de Barcelona Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. L.","family":"Sanz","sequence":"additional","affiliation":[{"name":"Clinica Universitaria de Navarra (CUN) Pamplona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Diaz\u2010Perales","sequence":"additional","affiliation":[{"name":"Centro de Biotecnologia y Genomica de Plantas Universidad Politecnica de Madrid\u2010Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"S\u00e1nchez\u2010L\u00f3pez","sequence":"additional","affiliation":[{"name":"Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS) Barcelona Spain"},{"name":"Unitat d'Al\u00b7lergia Servei de Pneumologia Hospital Clinic Universitat de Barcelona Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Garc\u00eda\u2010Moral","sequence":"additional","affiliation":[{"name":"Institut d'Investigacions Biomediques August Pi i Sunyer 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