{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T02:46:49Z","timestamp":1783133209267,"version":"3.54.6"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,5,26]],"date-time":"2016-05-26T00:00:00Z","timestamp":1464220800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HHSN272200700046C"],"award-info":[{"award-number":["HHSN272200700046C"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Allergies to cashew are increasing in prevalence, with clinical symptoms ranging from oral pruritus to fatal anaphylactic reaction. Yet, cashew\u2010specific T cell epitopes and T cell cross\u2010reactivity amongst cashew and other tree nut allergens in humans remain uncharacterized.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>In this study, we characterized cashew\u2010specific T cell responses in cashew\u2010allergic subjects and examined cross\u2010reactivity of these cashew\u2010specific cells towards other tree nut allergens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>154 up\u2010regulation assay was used to determine immunodominance hierarchy among cashew major allergens at the T cell level. The phenotype, magnitude and functionality of cashew\u2010specific T cells were determined by utilizing <jats:italic>ex\u00a0vivo<\/jats:italic> staining with <jats:styled-content style=\"fixed-case\">MHC<\/jats:styled-content> class <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> tetramers. Dual tetramer staining and proliferation experiments were used to determine cross\u2010reactivity to other tree nuts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> T cell responses were directed towards cashew allergens Ana o 1 and Ana o 2. Multiple Ana o 1 and Ana o 2 T cell epitopes were then identified. These epitopes elicited either <jats:styled-content style=\"fixed-case\">T<jats:sub>H<\/jats:sub><\/jats:styled-content>2 or <jats:styled-content style=\"fixed-case\">T<jats:sub>H<\/jats:sub><\/jats:styled-content>2\/<jats:styled-content style=\"fixed-case\">T<jats:sub>H<\/jats:sub><\/jats:styled-content>17 responses in allergic subjects, which were either cashew unique epitope or cross\u2010reactive epitopes. For clones that recognized the cross\u2010reactive epitope, T cell clones responded robustly to cashew, hazelnut and\/or pistachio but not to walnut.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Phylogenetically diverse tree nut allergens can activate cashew\u2010reactive T cells and elicit a <jats:styled-content style=\"fixed-case\">T<jats:sub>H<\/jats:sub><\/jats:styled-content>2\u2010type response at an epitope\u2010specific level.<\/jats:p><\/jats:sec><jats:sec><jats:title>Clinical relevance<\/jats:title><jats:p>Lack of cross\u2010reactivity between walnut and cashew suggests that cashew peptide immunotherapy approach may not be most effective for walnut.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.12746","type":"journal-article","created":{"date-parts":[[2016,4,30]],"date-time":"2016-04-30T22:44:58Z","timestamp":1462056298000},"page":"871-883","source":"Crossref","is-referenced-by-count":36,"title":["Ana o 1 and Ana o 2 cashew allergens share cross\u2010reactive <scp>CD<\/scp>4<sup>+<\/sup> T cell epitopes with other tree nuts"],"prefix":"10.1111","volume":"46","author":[{"given":"L. D.","family":"Archila","sequence":"first","affiliation":[{"name":"Benaroya Research Institute at Virginia Mason Seattle WA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I\u2010T.","family":"Chow","sequence":"additional","affiliation":[{"name":"Benaroya Research Institute at Virginia Mason Seattle WA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. W.","family":"McGinty","sequence":"additional","affiliation":[{"name":"Benaroya Research Institute at Virginia Mason Seattle WA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Renand","sequence":"additional","affiliation":[{"name":"Benaroya Research Institute at Virginia Mason Seattle WA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Jeong","sequence":"additional","affiliation":[{"name":"Virginia Mason Medical center Seattle WA USA"},{"name":"Department of Medicine University of Washington Seattle WA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Robinson","sequence":"additional","affiliation":[{"name":"Virginia Mason Medical center Seattle WA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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W.","family":"Kwok","sequence":"additional","affiliation":[{"name":"Benaroya Research Institute at Virginia Mason Seattle WA USA"},{"name":"Department of Medicine University of Washington Seattle WA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,5,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.03.029"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.115755"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/adc.2005.073817"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/pai.2009.0017"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1398-9995.2003.00342.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2007.01447.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2013.11.020"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12401"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2002.125208"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2005.02.028"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2009.12.009"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000072135"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2007.07.024"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3038.2005.00310.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1081-1206(10)61187-8"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.2332\/allergolint.10-OA-0222"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2008.02998.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2007.09.014"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI8476"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.8.4244"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.11.6665"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.01.029"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.03.037"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2009.03201.x"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/cea.12324"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/17.12.1236"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20101574"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.02.028"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2014.07.048"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2010.12.018"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.mn.7800196"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20011502"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80059-8"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2009.09.016"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.2332\/allergolint.08-OA-0010"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(76)90086-5"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.08.034"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20082901"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.09.014"}],"container-title":["Clinical &amp; Experimental Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcea.12746","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cea.12746","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T06:11:35Z","timestamp":1696313495000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cea.12746"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5,26]]},"references-count":39,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2016,6]]}},"alternative-id":["10.1111\/cea.12746"],"URL":"https:\/\/doi.org\/10.1111\/cea.12746","archive":["Portico"],"relation":{},"ISSN":["0954-7894","1365-2222"],"issn-type":[{"value":"0954-7894","type":"print"},{"value":"1365-2222","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,5,26]]}}}