{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:15:43Z","timestamp":1790709343179,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,6,6]],"date-time":"2016-06-06T00:00:00Z","timestamp":1465171200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81170022"],"award-info":[{"award-number":["81170022"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Airway eosinophilic inflammation is a pivotal feature of asthma. Epithelial cells play critical roles in airway eosinophilia. We hypothesized that epithelial micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s (mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s) are involved in airway eosinophilia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study investigated the associations between epithelial and plasma miR\u2010181b\u20105p and airway eosinophilic inflammation, and the possible mechanism by which miR\u2010181b\u20105p participates in eosinophilic inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Epithelial mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s expression was profiled by mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> array in eight subjects with asthma and four healthy controls. Epithelial miR\u2010181b\u20105p expression was confirmed by quantitative <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> in the subjects for array experiment and another cohort including 21 subjects with asthma and 10 controls. Plasma miR\u2010181b\u20105p was determined by quantitative <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> in 72 subjects with asthma and 35 controls. Correlation assays between epithelial and plasma miR\u2010181b\u20105p expression and airway eosinophilia were performed. The target of miR\u2010181b\u20105p, <jats:styled-content style=\"fixed-case\">SPP<\/jats:styled-content>1, was predicted by online algorithms and verified in <jats:styled-content style=\"fixed-case\">BEAS<\/jats:styled-content>\u20102B cells. The role of miR\u2010181b\u20105p in epithelial proinflammatory cytokine expression was examined in an <jats:italic>in vitro<\/jats:italic> system.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Epithelial miR\u2010181b\u20105p expression was decreased in subjects with asthma. Epithelial miR\u2010181b\u20105p levels were inversely correlated with sputum and bronchial submucosal eosinophilia. Plasma miR\u2010181b\u20105p was decreased and correlated with epithelial miR\u2010181b\u20105p in subjects with asthma. There was a strong inverse correlation between plasma miR\u2010181b\u20105p and airway eosinophilia in subjects with asthma. Plasma miR\u2010181b\u20105p was increased after inhaled corticosteroids treatment. We verified that <jats:styled-content style=\"fixed-case\">SPP<\/jats:styled-content>1 is a target of miR\u2010181b\u20105p. In human bronchial epithelial cells, miR\u2010181b\u20105p regulated <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201013\u2010induced <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2 and <jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>11 expression by targeting <jats:styled-content style=\"fixed-case\">SPP<\/jats:styled-content>1. Dexamethasone restored <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201013\u2010induced miR\u2010181b\u20105p down\u2010regulation and suppressed <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201013\u2010induced <jats:styled-content style=\"fixed-case\">SPP<\/jats:styled-content>1, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2 and <jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>11 expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p>Epithelial and plasma miR\u2010181b\u20105p are potential biomarkers for airway eosinophilia in asthma. MiR\u2010181b\u20105p may participate in eosinophilic airway inflammation by regulating proinflammatory cytokines expression via targeting <jats:styled-content style=\"fixed-case\">SPP<\/jats:styled-content>1.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.12754","type":"journal-article","created":{"date-parts":[[2016,5,18]],"date-time":"2016-05-18T14:19:34Z","timestamp":1463581174000},"page":"1281-1290","source":"Crossref","is-referenced-by-count":65,"title":["Decreased epithelial and plasma miR\u2010181b\u20105p expression associates with airway eosinophilic inflammation in asthma"],"prefix":"10.1111","volume":"46","author":[{"given":"X.","family":"Huo","sequence":"first","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine Department of Internal Medicine Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China"},{"name":"Key Laboratory of Respiratory Diseases National Health and Family Planning Commission of the People's 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