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The expression ofCD<\/jats:styled-content>8+<\/jats:sup>T\u2010cell subset relevant chemokines and chemokine receptors was detected by means of real\u2010timeRT<\/jats:styled-content>\u2010PCR<\/jats:styled-content>orELISA<\/jats:styled-content>. The cytotoxic activity of sortedCD<\/jats:styled-content>8+<\/jats:sup>T cells was defined by anti\u2010CD<\/jats:styled-content>3\u2010redirected killing assay.<\/jats:p><\/jats:sec>Results<\/jats:title>Compared with controls, elevated percentages of totalCD<\/jats:styled-content>8+<\/jats:sup>T cells and cytotoxic T lymphocyte (Tc) 1 (IFN<\/jats:styled-content>\u2010\u03b3+<\/jats:sup>), Tc2 (IL<\/jats:styled-content>\u20104+<\/jats:sup>), and Tc17 (IL<\/jats:styled-content>\u201017A+<\/jats:sup>) cell subset, and decreased percentages ofFOXP<\/jats:styled-content>3+<\/jats:sup>CD<\/jats:styled-content>8+<\/jats:sup>regulatory T cells, were found in both eosinophilic and non\u2010eosinophilic polyps with a Tc2\u2010skewed and Tc1\/Tc17\u2010dominated response in eosinophilic and non\u2010eosinophilic polyps, respectively. NasalCD<\/jats:styled-content>8+<\/jats:sup>T cells were found to produce similar or even higher levels ofIFN<\/jats:styled-content>\u2010\u03b3 andIL<\/jats:styled-content>\u20104 compared withCD<\/jats:styled-content>4+<\/jats:sup>T cells. Tc1 and Tc17, and Tc2 (IL<\/jats:styled-content>\u20104+<\/jats:sup>andIL<\/jats:styled-content>\u20105+<\/jats:sup>) cell subset percentages positively correlated with neutrophil and eosinophil counts in sinonasal mucosa, respectively. Strikingly, the expression of perforin and granzyme B and cytotoxic activity were significantly reduced in nasalCD<\/jats:styled-content>8+<\/jats:sup>T cells compared with their counterparts in peripheral blood. The expression ofCXCL<\/jats:styled-content>16,CCL<\/jats:styled-content>17, andCCL<\/jats:styled-content>20 positively correlated with Tc1, Tc2, and Tc17 cell subset number in sinonasal mucosa, respectively.<\/jats:p><\/jats:sec>Conclusion and Clinical Relevance<\/jats:title>CD<\/jats:styled-content>8+<\/jats:sup>T cells have low cytotoxic activity; nevertheless, they are a significant and previously underappreciated source of inflammatory cytokine production in polyps. Different Tc cell subset domination may contribute to distinctly biased granulocyte inflammation in eosinophilic and non\u2010eosinophilic polyps.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.12758","type":"journal-article","created":{"date-parts":[[2016,5,13]],"date-time":"2016-05-13T16:14:35Z","timestamp":1463156075000},"page":"1162-1175","source":"Crossref","is-referenced-by-count":52,"title":["CD<\/scp>8+<\/sup>T cells with distinct cytokine\u2010producing features and low cytotoxic activity in eosinophilic and non\u2010eosinophilic chronic rhinosinusitis with nasal polyps"],"prefix":"10.1111","volume":"46","author":[{"given":"J.","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 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