{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:32:25Z","timestamp":1791318745908,"version":"4.3.1"},"reference-count":32,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2017,9,15]],"date-time":"2017-09-15T00:00:00Z","timestamp":1505433600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009045","name":"Uniwersytet Jagiello\u0144ski Collegium Medicum","doi-asserted-by":"publisher","award":["K\/ZDS\/6164"],"award-info":[{"award-number":["K\/ZDS\/6164"]}],"id":[{"id":"10.13039\/100009045","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005297","name":"Uniwersytet Medyczny w Bialymstoku","doi-asserted-by":"publisher","award":["3\u201006616P"],"award-info":[{"award-number":["3\u201006616P"]}],"id":[{"id":"10.13039\/501100005297","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Arachidonic acid metabolites regulate several aspects of airway function including inflammation, muscle contraction and mucous secretion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>The aim of this study was to evaluate concentration of selected 5\u2010lipoxygenase\u2010 and cyclooxygenase\u2010derived eicosanoids in exhaled breath condensate (EBC) during allergen\u2010induced bronchoconstriction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The study was performed on 24 allergic rhinitis\/asthma patients sensitized to a house dust mite (HDM) <jats:italic>Dermatophagoides pteronyssinus<\/jats:italic> (Dp) and 13 healthy controls (HCs). Bronchial challenge with Dp extract was performed only in the allergic patients. EBC samples were collected before (<jats:italic>T<\/jats:italic><jats:sub>0<\/jats:sub>) and during Dp\u2010induced bronchoconstriction (<jats:italic>T<\/jats:italic><jats:sub>EAR<\/jats:sub>). Eicosanoid concentration was measured using HPLC\u2010tandem mass spectrometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Significant bronchoconstriction after Dp challenge was demonstrated in 15 patients (Rs), while in 9 patients (NRs) no asthmatic response could be detected. At <jats:italic>T<\/jats:italic><jats:sub>0<\/jats:sub> the most abundant eicosanoids in EBC of HDM\u2010allergic patients were LTB<jats:sub>4<\/jats:sub> and 5\u2010oxo\u2010ETE, while in HCs EBC concentration of LTB<jats:sub>4<\/jats:sub> was significantly greater than that of 5\u2010oxo\u2010ETE. Allergen challenge resulted in significant increase in EBC concentration of 5\u2010oxo\u2010ETE, LTD<jats:sub>4<\/jats:sub> and 8\u2010iso\u2010PGE<jats:sub>2<\/jats:sub> only in Rs. At <jats:italic>T<\/jats:italic><jats:sub>EAR<\/jats:sub>, the relative change of 5\u2010oxo\u2010ETE concentration in EBC correlated with decrease of peripheral blood eosinophilia (<jats:italic>R<\/jats:italic>\u00a0=\u00a0\u22120.774; <jats:italic>P<\/jats:italic>\u00a0=\u00a0.0012). Moreover, the relative increase of 5\u2010oxo\u2010ETE in EBC at <jats:italic>T<\/jats:italic><jats:sub>EAR<\/jats:sub> significantly correlated with the severity of the subsequent late asthmatic response (<jats:italic>R<\/jats:italic>\u00a0=\u00a00.683, <jats:italic>P<\/jats:italic>\u00a0=\u00a0.007).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our study demonstrates significant up\u2010regulation of 5\u2010oxo\u2010ETE synthesis in HDM\u2010allergic patients and indicates possible involvement of that mediator in the pathogenesis of allergic asthma.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.12990","type":"journal-article","created":{"date-parts":[[2017,8,1]],"date-time":"2017-08-01T14:39:56Z","timestamp":1501598396000},"page":"1253-1262","source":"Crossref","is-referenced-by-count":17,"title":["The effect of allergen\u2010induced bronchoconstriction on concentration of 5\u2010oxo\u2010ETE in exhaled breath condensate of house dust mite\u2010allergic 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