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Experimental systems for new\u2010onset\/chronic AD are needed to support rapid therapeutic development, particularly since animal models representing human AD are lacking. While HDM APT has been considered to simulate AD, its suitability to model AD's emerging Th2\/Th22 phenotype with Th1 and Th17 components is unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To assess whether HDM APT reproduces AD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Positive HDM APTs (n = 15) from patients with and without AD were evaluated, using genomic and immunohistochemistry studies, against intrapersonal control skin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>APT lesions showed higher T cell and dendritic cell infiltrates vs. controls. Seven hundred and forty\u2010three up\u2010 and 326 downregulated genes were differentially expressed in HDM APT (fold change &gt;2 and false discovery rate &lt; 0.05), with increased expression of Th2, Th9, Th17\/Th22 polar cytokines (i.e. IL\u20105, IL\u201013, IL\u20109, IL\u201017, IL\u201022).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>While HDM caused significant Th2 skewing, it also illustrated differences in Th2 induction and barrier defects; thus, HDM APT does not fully simulate AD. Given its widespread availability and sensitization rates, HDM may potentially be a useful tool that represents select aspects of AD, psoriasis, or contact dermatitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.13040","type":"journal-article","created":{"date-parts":[[2017,10,4]],"date-time":"2017-10-04T20:02:41Z","timestamp":1507147361000},"page":"1648-1660","source":"Crossref","is-referenced-by-count":25,"title":["Dust mite induces multiple polar T cell axes in human skin"],"prefix":"10.1111","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0590-6368","authenticated-orcid":false,"given":"K.","family":"Malik","sequence":"first","affiliation":[{"name":"Department of Dermatology Icahn School of Medicine at Mount Sinai New York NY USA"},{"name":"Laboratory for Investigative Dermatology The Rockefeller University New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Ungar","sequence":"additional","affiliation":[{"name":"Department of Dermatology Icahn School of Medicine at Mount Sinai New York NY USA"},{"name":"Laboratory for Investigative Dermatology The Rockefeller University New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Garcet","sequence":"additional","affiliation":[{"name":"Laboratory for Investigative Dermatology The Rockefeller University New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Dutt","sequence":"additional","affiliation":[{"name":"Department of Dermatology Icahn School of Medicine at Mount Sinai New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Dickstein","sequence":"additional","affiliation":[{"name":"Department of Dermatology Icahn School of Medicine at Mount Sinai New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X.","family":"Zheng","sequence":"additional","affiliation":[{"name":"Laboratory for Investigative Dermatology The Rockefeller University New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Dermatology Icahn School of Medicine at Mount Sinai New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y. 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