{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T21:50:50Z","timestamp":1792446650328,"version":"4.5.0"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T00:00:00Z","timestamp":1513900800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["SFB F4603"],"award-info":[{"award-number":["SFB F4603"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["W1248"],"award-info":[{"award-number":["W1248"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Buckwheat (<jats:italic>Fagopyrum esculentum<\/jats:italic>) has become increasingly popular as a healthy food in Europe. However, for sensitized individuals, consumption can cause anaphylactic reactions. The aim of this study was to identify individual well\u2010characterized buckwheat allergens for component\u2010resolved diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Patients were selected by positive skin prick test to buckwheat and divided into two groups: (1) sensitized to buckwheat without clinical symptoms and (2) buckwheat allergy. Buckwheat proteins were extracted from raw buckwheat seeds, purified applying a combination of protein precipitation and chromatographic methods, and analyzed by IgE immunoblotting and <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Buckwheat\u2010allergic patients had a significantly larger median skin prick test weal diameter for buckwheat than the sensitized group and the positive control. Also, IgE immunoblotting clearly showed a distinct pattern in sera from allergic patients when compared to sensitized individuals. Several IgE\u2010reactive proteins were purified from crude buckwheat extract, namely legumin (Fag\u00a0e\u00a01 plus its large subunit), Fag\u00a0e\u00a02 (2S albumin), and newly identified Fag e 5 (vicilin\u2010like) as well as hevein\u2010like antimicrobial peptides, designated Fag\u00a0e\u00a04. All four allergens showed superior diagnostic precision compared to extract\u2010based Immuno<jats:styled-content style=\"fixed-case\">CAP<\/jats:styled-content> with high sensitivity as well as high specificity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Patients with clinical symptoms clearly show a distinct allergen recognition pattern. We characterized a buckwheat vicilin\u2010like protein as a new relevant marker allergen, designated Fag e 5. Additionally, another new allergen, Fag\u00a0e\u00a04, potentially important for cross\u2010reactivity to latex was added to the allergen panel of buckwheat. Further, our data show that the full\u2010length legumin comprising both, large and small subunit should be applied for component\u2010resolved diagnosis. Our data indicate that concomitant sensitization to legumin, Fag e 2 and Fag e 5, predicts buckwheat allergy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.13068","type":"journal-article","created":{"date-parts":[[2017,11,24]],"date-time":"2017-11-24T14:52:47Z","timestamp":1511535167000},"page":"217-224","source":"Crossref","is-referenced-by-count":28,"title":["Concomitant sensitization to legumin, Fag e 2 and Fag e 5 predicts buckwheat allergy"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4749-3485","authenticated-orcid":false,"given":"S.","family":"Geiselhart","sequence":"first","affiliation":[{"name":"Department of Pathophysiology and Allergy Research Medical University of Vienna Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Nagl","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology and Allergy Research Medical University of Vienna Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Dubiela","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology and Allergy Research Medical University of Vienna Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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