{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T16:56:25Z","timestamp":1775580985443,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,6,11]],"date-time":"2018-06-11T00:00:00Z","timestamp":1528675200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003625","name":"Ministry of Health and Welfare","doi-asserted-by":"publisher","award":["HI13C0010"],"award-info":[{"award-number":["HI13C0010"]}],"id":[{"id":"10.13039\/501100003625","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003625","name":"Ministry of Health and Welfare","doi-asserted-by":"publisher","award":["HI14C1324"],"award-info":[{"award-number":["HI14C1324"]}],"id":[{"id":"10.13039\/501100003625","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Various allergenic proteins are produced by house dust mites (<jats:styled-content style=\"fixed-case\">HDM<\/jats:styled-content>). However, the allergenicity and clinical implications of these allergens are unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>The purpose of this study was to identify allergens in <jats:italic>Dermatophagoides farinae<\/jats:italic> and elucidate the sensitization profiles to these in Korean patients suffering from respiratory (allergic rhinitis and\/or asthma) and atopic dermatitis symptoms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>IgE reactivities in sera from 160 <jats:styled-content style=\"fixed-case\">HDM<\/jats:styled-content> allergy patients were analysed by one\u2010 and two\u2010dimensional gel electrophoresis and immunoblotting. IgE\u2010reactive components were identified by liquid chromatography\u2010coupled electrospray ionization\u2010tandem mass spectrometry. Nine recombinant mite allergens (Der f 1, Der f 2, Der f 10, Der f 11, Der f 13, Der f 14, Der f 30, Der f 32 and Der f Alt a 10) were produced, and the IgE reactivity in sera to each was determined by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>s.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Der f 1 and Der f 2 were recognized by IgE in serum samples from 88.1% and 78.1% of all patients, respectively. Patients with respiratory allergies were mainly sensitized to these major allergens, whereas patients with atopic dermatitis symptoms showed polysensitization to major and minor allergen components (including Der f 11, Der f 13, Der f 14, Der f 32 and Der f Alt a 10).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Patients with respiratory allergic disease sensitize to major allergen components of <jats:styled-content style=\"fixed-case\">HDM<\/jats:styled-content>. Those with atopic dermatitis were sensitized to a broader range of minor allergen components of <jats:styled-content style=\"fixed-case\">HDM<\/jats:styled-content> (Der f 11, Der f 13, Der f 14, Der f 32 and Der f Alt a 10).<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.13164","type":"journal-article","created":{"date-parts":[[2018,4,27]],"date-time":"2018-04-27T07:57:02Z","timestamp":1524815822000},"page":"1050-1058","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Sensitization to various minor house dust mite allergens is greater in patients with atopic dermatitis than in those with respiratory allergic disease"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3605-5364","authenticated-orcid":false,"given":"K. 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