{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T04:12:32Z","timestamp":1784952752007,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2018,9,14]],"date-time":"2018-09-14T00:00:00Z","timestamp":1536883200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["26461663"],"award-info":[{"award-number":["26461663"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>To date, three orange allergens have been reported. However, it is still unclear whether gibberellin\u2010regulated proteins (<jats:styled-content style=\"fixed-case\">GRP<\/jats:styled-content>s), identified as new allergens in other fruit allergies, are also involved in orange allergy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate the allergenicity of orange <jats:styled-content style=\"fixed-case\">GRP<\/jats:styled-content> and to determine the clinical characteristics of patients with orange allergy who are sensitized to orange <jats:styled-content style=\"fixed-case\">GRP<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We enrolled 14 patients (four men, 10 women, mean age: 29.6\u00a0years) who were diagnosed with orange allergy based on relevant clinical history, positive skin test, and\/or positive challenge test. Orange <jats:styled-content style=\"fixed-case\">GRP<\/jats:styled-content> (molecular weight: 6941.6\u00a0Da) was purified by ion\u2010exchange column chromatography. To test for orange <jats:styled-content style=\"fixed-case\">GRP<\/jats:styled-content>\u2010specific IgE, we performed <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>, basophil activation tests, and skin prick tests. Cross\u2010reactivity of orange <jats:styled-content style=\"fixed-case\">GRP<\/jats:styled-content> with native peach allergen <jats:styled-content style=\"fixed-case\">nP<\/jats:styled-content>ru p 7 and Japanese apricot <jats:styled-content style=\"fixed-case\">nP<\/jats:styled-content>ru m 7 was analysed by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> inhibition assays. IgE specific for orange, grapefruit, and peach allergens <jats:styled-content style=\"fixed-case\">rP<\/jats:styled-content>ru p 1, <jats:styled-content style=\"fixed-case\">rP<\/jats:styled-content>ru p 3, and <jats:styled-content style=\"fixed-case\">rP<\/jats:styled-content>ru p 4 was measured using ImmunoCAP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Twelve of the 14 patients (85.7%) were positive for orange <jats:styled-content style=\"fixed-case\">GRP<\/jats:styled-content> allergy in at least one test: 71.4% (10\/14) were positive by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>, 50% (3\/6) were positive in the basophil activation test, and 100% (4\/4) were positive in the skin prick test. <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> inhibition assays revealed cross\u2010reactivity of orange <jats:styled-content style=\"fixed-case\">GRP<\/jats:styled-content> with both <jats:styled-content style=\"fixed-case\">nP<\/jats:styled-content>ru p 7 and <jats:styled-content style=\"fixed-case\">nP<\/jats:styled-content>ru m 7. The patients showed variable positivity for specific IgE against orange, grapefruit, <jats:styled-content style=\"fixed-case\">rP<\/jats:styled-content>ru p 1, <jats:styled-content style=\"fixed-case\">rP<\/jats:styled-content>ru p 3, and <jats:styled-content style=\"fixed-case\">rP<\/jats:styled-content>ru p 4 (57.1%, 71.4%, 7.1%, 0%, and 21.4%, respectively). The most frequent symptoms of orange <jats:styled-content style=\"fixed-case\">GRP<\/jats:styled-content> allergy were facial swelling and oropharyngeal symptoms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p>Orange <jats:styled-content style=\"fixed-case\">GRP<\/jats:styled-content> may be involved in orange allergy and may be a cross\u2010reactive allergen between citrus fruits and the <jats:italic>Rosaceae<\/jats:italic> family of fruits.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.13247","type":"journal-article","created":{"date-parts":[[2018,8,12]],"date-time":"2018-08-12T21:38:01Z","timestamp":1534109881000},"page":"1509-1520","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["Identification of gibberellin\u2010regulated protein as a new allergen in orange 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