{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T13:08:56Z","timestamp":1785330536779,"version":"3.55.0"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,10,7]],"date-time":"2018-10-07T00:00:00Z","timestamp":1538870400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["TUNGER, PEI\u2010CBS\u201015"],"award-info":[{"award-number":["TUNGER, PEI\u2010CBS\u201015"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Common wheat (<jats:italic>Triticum aestivum<\/jats:italic>) and durum wheat (<jats:italic>T.\u00a0turgidum<\/jats:italic>) are both involved in Baker's asthma (BA) and food allergy (FA) including wheat\u2010dependent exercise\u2010induced asthma (WDEIA). However, allergens in durum wheat have not been described, and the over\u2010expression of <jats:italic>T.\u00a0turgidum<\/jats:italic> non\u2010specific lipid\u2010transfer protein (nsLTPs) is considered to increase resistance to phytopathogens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To identify and assess the allergenicity of nsLTP from <jats:italic>T.\u00a0turgidum<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Recombinant <jats:italic>T.\u00a0turgidum<\/jats:italic> nsLTP Tri tu 14 was generated and tested for structural integrity (circular dichroism\u2010spectroscopy) and purity (SDS\u2010PAGE). Thirty\u2010two wheat allergic patients were enrolled: 20 Spanish patients (BA) with positive bronchial challenge to wheat flour, and 12 Italian patients (wheat FA\/WDEIA) with positive double\u2010blind placebo\u2010controlled food challenge\/open food challenge (OFC) to pasta. IgE values to wheat, Tri tu 14, Tri a 14 (<jats:italic>T.\u00a0aestivum<\/jats:italic>) and Pru p 3 (<jats:italic>P.\u00a0persica<\/jats:italic>) were determined by ImmunoCAP testing. Allergenic potency (in\u00a0vitro mediator release) and IgE cross\u2010reactivity were investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Tri tu 14 was found to share 49% and 52% amino acid identity with Tri a 14 and Pru p 3, respectively. Among 25 Tri a 14 CAP positive sera, 23 (92%) were reactive to wheat extract, 22 (88%) to Tri tu 14 and 20 (80%) to Pru p 3. The correlation between Tri a 14 and Tri tu 14 specific IgE levels was <jats:italic>r<\/jats:italic>\u00a0=\u00a00.97 (BA) and <jats:italic>r<\/jats:italic>\u00a0=\u00a00.93 (FA\/WDEIA), respectively. FA\/WDEIA patients showed higher specific IgE values to Tri tu 14 and Pru p 3 than BA patients. Tri tu 14 displayed allergenic activity by mediator release from effector cells and IgE cross\u2010reactivity with Pru p 3. The degree of IgE cross\u2010reactivity between the two wheat nsLTPs varied between individual patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p>Sensitization to Tri tu 14 likely appears to be more important in wheat FA\/WDEIA than in BA. Over\u2010expression of Tri tu 14 in wheat would represent a risk for patients with nsLTP\u2010mediated FA.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.13271","type":"journal-article","created":{"date-parts":[[2018,9,10]],"date-time":"2018-09-10T17:54:24Z","timestamp":1536602064000},"page":"120-129","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Identification and molecular characterization of allergenic non\u2010specific lipid\u2010transfer protein from durum wheat (<i>Triticum turgidum<\/i>)"],"prefix":"10.1111","volume":"49","author":[{"given":"Hela","family":"Safi","sequence":"first","affiliation":[{"name":"Laboratory of Biotechnology and Plant Improvement Centre of Biotechnology Sfax Tunisia"},{"name":"Molecular Allergology Paul\u2010Ehrlich\u2010Institut Federal Institute for Vaccines and Biomedicines Langen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5184-5455","authenticated-orcid":false,"given":"Andrea","family":"Wangorsch","sequence":"additional","affiliation":[{"name":"Molecular Allergology Paul\u2010Ehrlich\u2010Institut Federal Institute for Vaccines and Biomedicines Langen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonas","family":"Lidholm","sequence":"additional","affiliation":[{"name":"Thermo Fisher Scientific Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fai\u00e7al","family":"Brini","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Plant Improvement Centre of Biotechnology Sfax Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jelena","family":"Spiric","sequence":"additional","affiliation":[{"name":"Division Allergology Paul\u2010Ehrlich\u2010Institut Federal Institute for Vaccines and Biomedicines Langen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans\u2010Peter","family":"Rihs","sequence":"additional","affiliation":[{"name":"Institute for Prevention and Occupational Medicine of the German Social Accident Insurance (IPA) Institute of the Ruhr\u2010University Bochum, Molecular Genetics Bochum Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Vieths","sequence":"additional","affiliation":[{"name":"Molecular Allergology Paul\u2010Ehrlich\u2010Institut Federal Institute for Vaccines and Biomedicines Langen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4165-4305","authenticated-orcid":false,"given":"Alicia","family":"Armentia","sequence":"additional","affiliation":[{"name":"Hospital Universitario R\u00edo Hortega Valladolid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Farioli","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine ASST Grande Ospedale Metropolitano Niguarda Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1093-3627","authenticated-orcid":false,"given":"Araceli","family":"Diaz\u2010Perales","sequence":"additional","affiliation":[{"name":"Departamento de Biotecnolog\u00eda\u2010Biolog\u00eda Vegetal E. 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