{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T01:16:09Z","timestamp":1790471769983,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,10,12]],"date-time":"2018-10-12T00:00:00Z","timestamp":1539302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002873","name":"Chulalongkorn University","doi-asserted-by":"publisher","award":["GRB\u2010BSS_83_59_30_24"],"award-info":[{"award-number":["GRB\u2010BSS_83_59_30_24"]}],"id":[{"id":"10.13039\/501100002873","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["083625"],"award-info":[{"award-number":["083625"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Protein crystallographic studies suggest that the house dust mite (HDM) allergen Der p 5 potentially interacts with hydrophobic ligands. Der p 5, in association with its ligand(s), might therefore trigger innate immune signalling pathways in the airway epithelium and influence the initiation of the HDM\u2010allergic response.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>We investigated the lipid binding propensities of recombinant (r)Der p 5 and characterized the signalling pathways triggered by the allergen in airway epithelial cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>rDer p 5 was produced in <jats:italic>Pichia pastoris<\/jats:italic> and characterized by mass spectrometry, multi\u2010angle light scattering and circular dichroism. Its interactions with hydrophobic ligands were investigated in fluorescence\u2010based lipid binding assays and in\u2010silico docking simulations. Innate immune signalling pathways triggered by rDer p 5 were investigated in airway epithelial cell activation assays in\u00a0vitro.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Biophysical analysis showed that rDer p 5 was monomeric and adopted a similar \u03b1\u2010helix\u2010rich fold at both physiological and acidic pH. Spectrofluorimetry experiments showed that rDer p 5 is able to selectively bind lipid ligands, but only under mild acidic pH conditions. Computer\u2010based docking simulations identified potential binding sites for these ligands. This allergen, with putatively associated lipid(s), triggered the production of IL\u20108 in respiratory epithelial cells through a TLR2\u2010, NF\u2010kB\u2010 and MAPK\u2010dependent signalling pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p>Despite the fact that Der p 5 represents a HDM allergen of intermediate prevalence, our findings regarding its lipid binding and activation of TLR2 indicate that it could participate in the initiation of the HDM\u2010allergic state.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.13278","type":"journal-article","created":{"date-parts":[[2018,9,19]],"date-time":"2018-09-19T11:44:55Z","timestamp":1537357495000},"page":"378-390","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["The house dust mite allergen Der p 5 binds lipid ligands and stimulates airway epithelial cells through a TLR2\u2010dependent pathway"],"prefix":"10.1111","volume":"49","author":[{"given":"Pinya","family":"Pulsawat","sequence":"first","affiliation":[{"name":"Faculty of Medicine Chula Vaccine Research Center Chulalongkorn University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tewarit","family":"Soongrung","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Chula Vaccine Research Center Chulalongkorn University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pattraporn","family":"Satitsuksanoa","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Chula Vaccine Research Center Chulalongkorn University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxime","family":"Le\u00a0Mignon","sequence":"additional","affiliation":[{"name":"Stallergenes Greer Antony 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