{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,7]],"date-time":"2025-06-07T08:45:17Z","timestamp":1749285917501,"version":"3.37.3"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,10,12]],"date-time":"2018-10-12T00:00:00Z","timestamp":1539302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Investigating disease mechanisms and treatment responses in obstructive airway diseases with invasive sampling are hampered by the small size and mechanical artefacts that conventional forceps biopsies suffer from. Endoscopic cryobiopsies are larger and more intact and are being increasingly used. However, the technique has not yet been explored for obtaining mucosa biopsies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate differences in size and quality of endobronchial mucosal biopsies obtained with cryotechnique and forceps. Further, to check for eligibility of cryobiopsies to be evaluated with immunohistochemistry and in\u00a0situ hybridization and to investigate tolerability and safety of the technique.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Endobronchial mucosal biopsies were obtained with cryotechnique and forceps from patients with haemoptysis undergoing bronchoscopy and evaluated by quantitative morphometry, automated immunohistochemistry and in\u00a0situ hybridization.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 40 biopsies were obtained from 10 patients. Cross\u2010sectional areas were threefold larger in cryobiopsies (median: 3.08\u00a0mm<jats:sup>2<\/jats:sup> (<jats:styled-content style=\"fixed-case\">IQR<\/jats:styled-content>: 1.79) vs 1.03\u00a0mm<jats:sup>2<\/jats:sup> (<jats:styled-content style=\"fixed-case\">IQR<\/jats:styled-content>: 1.10), <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001). Stretches of intact epithelium were 8\u2010fold longer (median: 4.61\u00a0mm (<jats:styled-content style=\"fixed-case\">IQR<\/jats:styled-content>: 4.50) vs 0.55\u00a0mm (<jats:styled-content style=\"fixed-case\">IQR<\/jats:styled-content>: 1.23), <jats:italic>P<\/jats:italic>\u00a0=\u00a00.001). Content of glands (median: 0.095\u00a0mm<jats:sup>2<\/jats:sup> (<jats:styled-content style=\"fixed-case\">IQR<\/jats:styled-content>: 0.30) vs 0.00\u00a0mm<jats:sup>2<\/jats:sup> (<jats:styled-content style=\"fixed-case\">IQR<\/jats:styled-content>: 0.01), <jats:italic>P<\/jats:italic>\u00a0=\u00a00.002) and airway smooth muscle (median: 0.25\u00a0mm<jats:sup>2<\/jats:sup> (<jats:styled-content style=\"fixed-case\">IQR<\/jats:styled-content>: 0.30) vs 0.060\u00a0mm<jats:sup>2<\/jats:sup> (<jats:styled-content style=\"fixed-case\">IQR<\/jats:styled-content>: 0.11), <jats:italic>P<\/jats:italic>\u00a0=\u00a00.02) was higher in the cryobiopsies compared with forceps biopsies. Further, the cryobiopsies had well\u2010preserved protein antigens and <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>. Mild to moderate bleeding was the only complication observed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion and clinical relevance<\/jats:title><jats:p>By yielding significantly larger and more intact biopsies, the cryotechnique represents a valuable new research tool to explore the bronchi in airway disease. Ultimately with the potential to create better understanding of underlying disease mechanisms and improvement of treatments.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.13281","type":"journal-article","created":{"date-parts":[[2018,9,23]],"date-time":"2018-09-23T08:23:43Z","timestamp":1537691023000},"page":"27-34","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Bronchoscopic mucosal cryobiopsies as a method for studying airway disease"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7995-2965","authenticated-orcid":false,"given":"Morten","family":"Hvidtfeldt","sequence":"first","affiliation":[{"name":"Respiratory Research Unit Bispebjerg Hospital Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexis","family":"Pulga","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Bispebjerg Hospital Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morten","family":"Hostrup","sequence":"additional","affiliation":[{"name":"Respiratory Research Unit Bispebjerg Hospital Copenhagen Denmark"},{"name":"Department of Nutrition, Exercise and Sport University of Copenhagen Copenhagen \u00d8 Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline","family":"Sanden","sequence":"additional","affiliation":[{"name":"Medetect AB Lund Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michiko","family":"Mori","sequence":"additional","affiliation":[{"name":"Unit of Airway Inflammation Lund University Lund Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisy","family":"Bornesund","sequence":"additional","affiliation":[{"name":"Unit of Airway Inflammation Lund University Lund Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus R.","family":"Larsen","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Bispebjerg Hospital Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6716-3010","authenticated-orcid":false,"given":"Jonas S.","family":"Erjef\u00e4lt","sequence":"additional","affiliation":[{"name":"Unit of Airway Inflammation Lund University Lund Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4825-9436","authenticated-orcid":false,"given":"Celeste","family":"Porsbjerg","sequence":"additional","affiliation":[{"name":"Respiratory Research Unit Bispebjerg Hospital Copenhagen Denmark"},{"name":"Department of Respiratory Medicine Bispebjerg Hospital Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3109\/02770903.2014.999283"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.122.6.1909"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/thx.51.7.664"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.157.3.9705020"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200202-150WS"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201505-0963WS"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2008\/202615"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1513\/pats.200705-063VS"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201504-0711OC"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/crj.12261"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000444089"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/resp.12309"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.4103\/1817-1737.69117"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00033011"},{"key":"e_1_2_7_16_1","unstructured":"American Society of Anesthesiologists. 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