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A basophil CD<\/jats:styled-content>63 response to in vitro stimulation with Dermatophagoides\u00a0pteronyssinus<\/jats:italic> whole allergen extract and the IgE reactivity profiles for Der p 1, 2, 4, 5, 7, 10, 11, 14, 15, 18, 21, 23 were evaluated. Serum IgE and IgG specific to D\u00a0pteronyssinus<\/jats:italic> whole allergen extract and total IgE were measured.<\/jats:p><\/jats:sec>Results<\/jats:title>There were no statistically significant differences in the levels of IgE (IgE levels were higher in symptomatic patients with P\u00a0<\/jats:italic>=\u00a0<\/jats:italic>0.055) and IgG specific to D\u00a0pteronyssinus<\/jats:italic> and total IgE. Symptomatic patients showed a lower threshold for in vitro basophil activation (3.33\u00a0ng\/mL<\/jats:styled-content> vs 33.3\u00a0ng\/mL<\/jats:styled-content>), a higher area under the curve (AUC<\/jats:styled-content>) of basophil activation (171 vs 127) (P\u00a0<\/jats:italic>=\u00a0<\/jats:italic>0.017), a higher response to positive control with anti\u2010Fc\u03b5RI<\/jats:styled-content> stimulation (97% vs 79%) (P\u00a0<\/jats:italic><\u00a0<\/jats:italic>0.001), a recognition of more HDM<\/jats:styled-content> allergens (4 vs 2) and more frequent sensitization to rD<\/jats:styled-content>er p 7 (P\u00a0=\u00a0<\/jats:italic>0.016) and rD<\/jats:styled-content>er p 23 compared to asymptomatic subjects (P\u00a0<\/jats:italic>=\u00a0<\/jats:italic>0.018). There was a positive correlation (r<\/jats:italic>\u00a0=\u00a00.63; P\u00a0<\/jats:italic><\u00a0<\/jats:italic>0.001) between the number of recognized allergens and the AUC<\/jats:styled-content> of basophil activation.<\/jats:p><\/jats:sec>Conclusion and Clinical Relevance<\/jats:title>In the subjects studied, the differences in the basophil response to D\u00a0pteronyssinus<\/jats:italic> allergen extract, number of recognized HDM<\/jats:styled-content> allergens and reactivity to rD<\/jats:styled-content>er p 7 and rD<\/jats:styled-content>er p 23 distinguish symptomatic from asymptomatic HDM<\/jats:styled-content> sensitisation better than SPT<\/jats:styled-content> or allergen extract\u2010specific IgE. Information regarding the clinical relevance of sensitization is important for the prescription of allergen\u2010specific immunotherapy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.13361","type":"journal-article","created":{"date-parts":[[2019,2,8]],"date-time":"2019-02-08T06:21:00Z","timestamp":1549606860000},"page":"808-818","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Clinical and immunological differences between asymptomatic HDM<\/scp>\u2010sensitized and HDM<\/scp>\u2010allergic rhinitis patients"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0515-5207","authenticated-orcid":false,"given":"Mihaela","family":"Zidarn","sequence":"first","affiliation":[{"name":"University Clinic of Pulmonary and Allergic Diseases Golnik Golnik Slovenia"},{"name":"Faculty of Medicine University of Ljubljana Ljubljana 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