{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T03:20:43Z","timestamp":1790047243252,"version":"4.0.1"},"reference-count":37,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,6,5]],"date-time":"2019-06-05T00:00:00Z","timestamp":1559692800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01-AI099029"],"award-info":[{"award-number":["R01-AI099029"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R21-AI135397"],"award-info":[{"award-number":["R21-AI135397"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>2S\u2010albumins Ara h 2 and Ara h 6 are the most potent peanut allergens and levels of specific immunoglobulin E (IgE) towards these proteins are good predictors of clinical reactivity. Because of structural homologies, Ara h 6 is generally considered to cross\u2010react extensively with Ara h 2.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>We aimed to quantify the IgE cross\u2010reactivity between Ara h 2 and Ara h 6.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Peanut 2S\u2010albumins were purified from raw peanuts. The IgE cross\u2010reactivity between Ara h 2 and Ara h 6 was evaluated with 32 sera from French and US peanut\u2010allergic patients by measuring the residual IgE\u2010binding to one 2S\u2010albumin after depletion of IgE antibodies recognizing the other 2S\u2010albumin. The IgE cross\u2010reactivity between Ara h 2 and Ara h 6 was further investigated by competitive inhibition of IgE\u2010binding and by a model of mast cell degranulation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A highly variable level of IgE cross\u2010reactivity was revealed among the patients. The mean fraction of cross\u2010reactive IgE antibodies represented only 17.1% of 2S\u2010albumins\u2010specific IgE antibodies and was lower than the mean fraction of IgE specific to Ara h 2 (57.4%) or to Ara h 6 (25.5%). The higher level of Ara h 2\u2010specific IgE was principally due to the IgE\u2010binding capacity of an insertion containing the repeated immunodominant linear epitope DPYSP<jats:sup>OH<\/jats:sup>S. The impact of IgE cross\u2010reactivity on diagnostic testing was illustrated with a serum displaying an Ara h 6\u2010specific IgE response of 26 UI\/mL that was not associated with the capacity of Ara h 6 to trigger mast cell degranulation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Clinical Relevance<\/jats:title><jats:p>Immunoglobulin E antibodies specific to peanut 2S\u2010albumins are mainly non\u2013cross\u2010reactive, but low\u2010affinity cross\u2010reactivity can affect diagnostic accuracy. Testing IgE\u2010binding to a mixture of 2S\u2010albumins rather than to each separately may enhance diagnostic performance.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.13432","type":"journal-article","created":{"date-parts":[[2019,5,20]],"date-time":"2019-05-20T17:34:01Z","timestamp":1558373641000},"page":"1107-1115","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Variable IgE cross\u2010reactivity between peanut 2S\u2010albumins: The case for measuring IgE to both Ara h 2 and Ara h 6"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4828-7031","authenticated-orcid":false,"given":"St\u00e9phane","family":"Hazebrouck","sequence":"first","affiliation":[{"name":"Service de Pharmacologie et Immunoanalyse (SPI), Laboratoire d'Immuno\u2010Allergie Alimentaire CEA, INRA, Universit\u00e9 Paris\u2010Saclay Gif\u2010sur\u2010Yvette 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