{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T20:01:52Z","timestamp":1775592112096,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,10,6]],"date-time":"2019-10-06T00:00:00Z","timestamp":1570320000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670019, 91742108"],"award-info":[{"award-number":["81670019, 91742108"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>microRNA (miR)\u2010218\u20105p is involved in cigarette smoke\u2010induced airway inflammation. In our earlier asthma epithelial miRNA profiling data, miR\u2010218\u20105p was the top 2 down\u2010regulated miRNA. We hypothesize that miR\u2010218\u20105p plays a role in asthma airway inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To unveil the role of miR\u2010218\u20105p and its target gene in asthma airway inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We measured miR\u2010218\u20105p expression in bronchial brushings of asthma patients (n\u00a0=\u00a050) and healthy controls (n\u00a0=\u00a015), and analysed the correlations between miR\u2010218\u20105p expression and airway eosinophilia. We examined whether <jats:italic>CTNND2<\/jats:italic> was a target of miR\u2010218\u20105p, and the expression of 12 catenin family members in bronchial brushings, in cultured human bronchial epithelial (HBE) cells and BEAS\u20102B cells. We explored the role of miR\u2010218\u20105p\u2010CTNND2 pathway using a murine model of allergic airway inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Epithelial miR\u2010218\u20105p expression was significantly decreased and negatively correlated with eosinophils in induced sputum and bronchial biopsies, and other type 2 biomarkers in asthma patients. We verified that <jats:italic>CTNND2<\/jats:italic> (encoding \u03b4\u2010catenin) was a target of miR\u2010218\u20105p. Remarkably, CTNND2 was the most significantly up\u2010regulated catenin compared with the other 11 catenin family members in bronchial brushings of asthma patients, IL\u201013\u2010stimulated HBE and BEAS\u20102B cells. Moreover, epithelial CTNND2 expression positively correlated with airway eosinophilia in asthma. Airway mmu\u2010miR\u2010218\u20105p expression was also decreased, and Ctnnd2 expression was increased in a murine model of allergic airway inflammation. Intriguingly, mmu\u2010miR\u2010218\u20105p overexpression suppressed airway hyperresponsiveness, eosinophilic airway inflammation and Ctnnd2 up\u2010regulation in the mouse model. Finally, perturbation of miR\u2010218\u20105p or CTNND2 expression significantly altered chemokine CCL26 expression in the cell cultures and the mouse model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p>Epithelial miR\u2010218\u20105p plays a protective role in eosinophilic airway inflammation via targeting CTNND2, a novel catenin in asthma, and suppressing chemokine CCL26 expression.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.13498","type":"journal-article","created":{"date-parts":[[2019,9,14]],"date-time":"2019-09-14T02:32:52Z","timestamp":1568428372000},"page":"29-40","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["microRNA\u2010218\u20105p plays a protective role in eosinophilic airway inflammation via targeting \u03b4\u2010catenin, a novel catenin in 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