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It is clinically characterized by dysphagia, food impaction and oesophageal eosinophilia. Epidemiological studies indicate that obesity can worsen allergic symptoms; however, its effect on EoE immunopathological response has not been evaluated yet. This study aimed to assess the effect of obesity on allergic inflammation and T helper\u20102 profile in an EoE experimental model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Obesity was induced by high\u2010fat feeding. After 7\u00a0weeks of diet, male BALB\/c mice were subcutaneously sensitized and orally challenged with OVA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Obesity itself induced a significant mast cell and eosinophil accumulation in the oesophagus, trachea, gut and lung. After allergy induction, this number was higher, when compared to lean\u2010allergic mice. Moreover, obese\u2010allergic mice showed higher remodelling area, in the oesophagus, associated with higher <jats:italic>IL\u20105<\/jats:italic> and <jats:italic>TSLP<\/jats:italic> mRNA expression. In contrast, <jats:italic>FoxP3<\/jats:italic> and <jats:italic>IL\u201010<\/jats:italic> were less expressed in comparison with lean\u2010allergic mice. In addition, the amount of CD11c<jats:sup>+<\/jats:sup>MHCII<jats:sup>+<\/jats:sup>PDL1<jats:sup>+<\/jats:sup> dendritic cells was reduced, while the number of CD11c<jats:sup>+<\/jats:sup>MHCII<jats:sup>+<\/jats:sup>CD80<jats:sup>+<\/jats:sup> DCs and CD3<jats:sup>+<\/jats:sup>CD4<jats:sup>+<\/jats:sup>GATA<jats:sub>3<\/jats:sub><jats:sup>+<\/jats:sup>IL\u20104<jats:sup>+<\/jats:sup> cells was increased in obese\u2010allergic mice in the spleen and lymph nodes when compared to lean\u2010allergic mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Obesity aggravated the immune histopathological characteristics in the EoE experimental model, which was associated with the reduction in the regulatory profile, and the increased inflammatory cells influx, related to the T<jats:sub>H<\/jats:sub>2 profile. Altogether, the data provide new knowledge about obesity as a risk factor, worsening EoE symptoms, and contribute for future treatment strategies for this specific profile.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.13533","type":"journal-article","created":{"date-parts":[[2019,12,14]],"date-time":"2019-12-14T14:30:25Z","timestamp":1576333825000},"page":"244-255","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["High\u2010fat diet\u2010induced obesity worsens TH2 immune response and immunopathologic characteristics in murine model of eosinophilic oesophagitis"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5294-8376","authenticated-orcid":false,"given":"Fl\u00e1via M\u00e1rcia de Castro e","family":"Silva","sequence":"first","affiliation":[{"name":"Department of Parasitology, Microbiology and Immunology Institute of Biological Sciences Federal University of Juiz de Fora Juiz de 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