{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:28:09Z","timestamp":1787243289011,"version":"build-2736575974"},"reference-count":48,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,12,14]],"date-time":"2019-12-14T00:00:00Z","timestamp":1576281600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001060","name":"Asthma Foundation of Western Australia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003748","name":"Svenska S\u00e4llskapet f\u00f6r Medicinsk Forskning","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003748","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The prevalence and severity of asthma, particularly the most common (atopic) form of the disease, increase amongst females but not males after puberty, and asthma activity also changes throughout the menstrual cycle and during pregnancy. The contribution of female sex hormones to asthma pathogenesis is incompletely understood.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To obtain insight into the role of oestrogen (E2) in experimental atopic asthma, and guide future research on sex\u2010related variations in atopic asthma susceptibility\/intensity in humans.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We utilized an experimental model comprising rat strains expressing dichotomous Th2\u2010high vs Th2\u2010low immunophenotypes exemplified by eosinophilia, mirroring differences between human atopics\/non\u2010atopics. We compared the efficiency of Th2\u2010associated immunoinflammatory mechanisms, which differed markedly between the two strains, and between sexes in the Th2\u2010high strain, and determined the effects of E2 administration on these differences.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Unique to the Th2\u2010high strain, eosinophil: neutrophil ratios in the airways at baseline and following sensitization\/aeroallergen challenge were logfold higher in females relative to males, and this was reflected by higher baseline blood eosinophil numbers in females. Pretreatment of Th2\u2010high males with E2 abrogated this sex difference by selectively boosting Th2\u2010associated genes in the airways and eosinophilia, but was without corresponding effect in the Th2\u2010low strain. In contrast, parallel E2 effects on myeloid and lymphoid cell populations were relatively modest.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Clinical Relevance<\/jats:title>\n                    <jats:p>E2 acts to amplify the eosinophilic component of pre\u2010existing Th2\u2010high immunophenotype, possibly acting at the level of the common eosinophil\/neutrophil precursor in bone marrow to preferentially drive eosinophil differentiation. Constitutive granulocyte profiles in which the balance between eosinophils and neutrophils is skewed towards eosinophils have been identified in independent cohort studies as markers of asthma risk, and these findings suggest that more detailed studies on the role of E2 in this context, and in relation to asthma pathogenesis in post\u2010pubertal females in particular, appear warranted.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cea.13544","type":"journal-article","created":{"date-parts":[[2019,12,2]],"date-time":"2019-12-02T05:22:51Z","timestamp":1575264171000},"page":"391-400","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Oestrogen amplifies pre\u2010existing atopy\u2010associated Th2 bias in an experimental asthma model"],"prefix":"10.1111","volume":"50","author":[{"given":"Jean Francois","family":"Lauzon\u2010Joset","sequence":"first","affiliation":[{"name":"Telethon Kids Institute University of Western Australia Nedlands WA Australia"},{"name":"Centre de Recherche Institut Universitaire de Cardiologie et de Pneumologie de Qu\u00e9bec Universit\u00e9 Laval Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyle T.","family":"Mincham","sequence":"additional","affiliation":[{"name":"Telethon Kids Institute University of Western Australia Nedlands WA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana P.","family":"Abad","sequence":"additional","affiliation":[{"name":"Telethon Kids Institute University of Western Australia Nedlands WA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Braden P.","family":"Short","sequence":"additional","affiliation":[{"name":"Telethon Kids Institute University of Western Australia Nedlands WA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick G.","family":"Holt","sequence":"additional","affiliation":[{"name":"Telethon Kids Institute University of Western Australia Nedlands WA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah H.","family":"Strickland","sequence":"additional","affiliation":[{"name":"Telethon Kids Institute University of Western Australia Nedlands WA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5674-8462","authenticated-orcid":false,"given":"Jonatan","family":"Leffler","sequence":"additional","affiliation":[{"name":"Telethon Kids Institute University of Western Australia Nedlands WA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,12,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/ACI.0b013e32835a6dd6"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1081-1206(10)61063-0"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2003.1676"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.4168\/aair.2011.3.2.74"},{"key":"e_1_2_8_6_1","doi-asserted-by":"crossref","unstructured":"MelgertBN RayA HylkemaMN TimensW PostmaDS. 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