{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T12:01:34Z","timestamp":1772712094667,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,2,14]],"date-time":"2020-02-14T00:00:00Z","timestamp":1581638400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002722","name":"AllerGen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002722","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Genome\u2010wide association studies have identified associations of the single nucleotide polymorphism rs1837253 in the thymic stromal lymphopoietin (<jats:italic>TSLP<\/jats:italic>) gene with asthma, allergic disease and eosinophilia. The <jats:italic>TSLP<\/jats:italic> gene encodes two isoforms, long and short, and previous studies have indicated functional differences between these two isoforms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>We investigated the expression of these TSLP isoforms in response to a pro\u2010inflammatory signal, and the role of the rs1837253 genotype in gene isoform regulation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We cultured nasal epithelial cells of asthmatic and non\u2010asthmatic subjects and evaluated poly(I:C)\u2010induced TSLP protein secretion using multiplex protein assays and gene expression profiles of the TSLP isoforms, and related genes using real\u2010time qPCR. We correlated these profiles with rs1837253 genotype.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Asthmatic nasal epithelial cells exhibited increased TSLP protein secretion compared with nasal epithelial cells from healthy controls. The long TSLP isoform was more responsive to poly(I:C) stimulation. Additionally, the minor T allele of rs1837253 was less inducible than the major C allele, suggesting differential regulation; this may explain the \u201cprotective\u201d effects of the T allele in asthma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results provide important insights into the differential regulation and function of TSLP isoforms, including the role of <jats:italic>TSLP<\/jats:italic> rs1837253 polymorphisms in allergic inflammatory processes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Clinical relevance<\/jats:title><jats:p>The key finding on the influence of TSLP genetic variation on disease expression\/endotype could provide basis for investigation into targeted biologics for anti\u2010TSLP therapies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.13568","type":"journal-article","created":{"date-parts":[[2020,1,13]],"date-time":"2020-01-13T14:20:32Z","timestamp":1578925232000},"page":"471-478","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["A thymic stromal lymphopoietin polymorphism may provide protection from asthma by altering gene expression"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0805-6821","authenticated-orcid":false,"given":"Amy","family":"Moorehead","sequence":"first","affiliation":[{"name":"Department of Medicine McMaster University Hamilton ON Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raphael","family":"Hanna","sequence":"additional","affiliation":[{"name":"Department of Medicine McMaster University Hamilton ON 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