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Sublingual immunotherapy (SLIT) shows an excellent safety profile for food allergy, but the clinical efficacy needs to be improved. This study assessed the effects of the Toll\u2010like receptor 4 agonist outer membrane protein (Omp) 16 from <jats:italic>Brucella abortus<\/jats:italic> combined with cow\u00b4s milk proteins (CMP) through the sublingual route to modulate cow's milk allergy in an experimental model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mice sensitized with cholera toxin and CMP were orally challenged with the allergen to elicit hypersensitivity reactions. Then, mice were treated with a very low amount of CMP along with Omp16 as a mucosal adjuvant, and finally, animals were re\u2010exposed to CMP. Systemic and mucosal immune parameters were assessed in vivo and in vitro.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that the sublingual administration of Omp16\u00a0+\u00a0CMP induced a buccal Th1 immune response that modulated the intestinal allergic response with the suppression of symptoms, reduction of IgE and IL\u20105, and up\u2010regulation of IgG2a and IFN\u2010\u03b3. The adoptive transfer of submandibular IFN\u2010\u03b3\u2010producing \u03b14\u03b27<jats:sup>+<\/jats:sup>CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> cells conferred protection against allergic sensitization. The use of Omp16\u00a0+\u00a0CMP promoted enhanced protection compared to CMP alone.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In conclusion, Omp16 represents a promising mucosal adjuvant that can be used to improve the clinical and immune efficacy of SLIT for food allergy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.13676","type":"journal-article","created":{"date-parts":[[2020,6,5]],"date-time":"2020-06-05T14:35:43Z","timestamp":1591367743000},"page":"954-963","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Sublingual Omp16\u2010driven redirection of the allergic intestinal response in a pre\u2010clinical model of food allergy"],"prefix":"10.1111","volume":"50","author":[{"given":"Maria Luc\u00eda","family":"Orsini Delgado","sequence":"first","affiliation":[{"name":"Instituto de Estudios Inmunol\u00f3gicos y Fisiopatol\u00f3gicos (IIFP), UNLP, CONICET, asociado a CIC PBA, Facultad de Ciencias Exactas, Departamento de Ciencias Biol\u00f3gicas La Plata Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gast\u00f3n Pascual","family":"Rizzo","sequence":"additional","affiliation":[{"name":"Instituto de Estudios Inmunol\u00f3gicos y Fisiopatol\u00f3gicos (IIFP), UNLP, CONICET, asociado a CIC PBA, Facultad de Ciencias Exactas, Departamento de Ciencias Biol\u00f3gicas La Plata Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Alberto","family":"Fossati","sequence":"additional","affiliation":[{"name":"Instituto de Estudios Inmunol\u00f3gicos y Fisiopatol\u00f3gicos (IIFP), UNLP, CONICET, asociado a CIC PBA, Facultad de Ciencias Exactas, Departamento de Ciencias Biol\u00f3gicas La Plata Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karina Alejandra","family":"Pasquevich","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biotecnol\u00f3gicas \u201cDr. Rodolfo A. 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