{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T11:48:55Z","timestamp":1780400935006,"version":"3.54.1"},"reference-count":18,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,4,9]],"date-time":"2021-04-09T00:00:00Z","timestamp":1617926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Furry animals are an important source of indoor allergens. Diagnosis of allergy to small pets such as guinea\u2010pigs still relies on animal dander extracts which do not allow to define the primary sensitization source.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To identify major guinea\u2010pig allergens and to evaluate their potential as marker allergens for in vitro IgE\u2010diagnosis in comparison with dander extracts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A group of patients allergic to guinea\u2010pig (<jats:italic>n<\/jats:italic>\u00a0=\u00a029) and a group of patients allergic to cat and dog (<jats:italic>n<\/jats:italic>\u00a0=\u00a030) were recruited for the study. A panel of four guinea\u2010pig lipocalin allergens was expressed as recombinant proteins in <jats:italic>E<\/jats:italic>.\u00a0<jats:italic>coli<\/jats:italic>. Specific IgE were quantified by ImmunoCAP and ELISA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The combination of 4 guinea\u2010pig lipocalin allergens, including 2 new lipocalins, Cav p 1.0201 and Cav p 6.0101, and the previously characterized lipocalins Cav p 2 and Cav p 3, enabled the identification of 90% of all patients allergic to guinea\u2010pig. The vast majority had specific IgE to Cav p 1 (83%). Cav p 6 shares 54% sequence identity with Fel d 4 and Can f 6 and was found to be IgE\u2010cross\u2010reactive with these allergens. In the group of cat\u2010 and dog\u2010allergic patients, 73% had also specific IgE to guinea\u2010pig dander. However, only 27% of the cat \/dog\u2010allergic patients had specific IgE to any of the non\u2010cross\u2010reactive guinea\u2010pig allergens Cav p 1, Cav p 2 or Cav p 3. The high prevalence of IgE to guinea\u2010pig dander could be explained by IgE\u2010cross\u2010reactivity among serum albumins and certain lipocalins.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical relevance<\/jats:title><jats:p>The availability of specific allergen markers is essential for the assessment of primary sensitization, especially in polysensitized patients. The proposed panel of guinea\u2010pig allergens Cav p 1, Cav p 2 and Cav p 3 is a first step to component\u2010resolved IgE\u2010diagnosis of allergy to small furry pets.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.13873","type":"journal-article","created":{"date-parts":[[2021,4,2]],"date-time":"2021-04-02T07:15:10Z","timestamp":1617347710000},"page":"829-835","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Component\u2010resolved diagnosis using guinea\u2010pig allergens elucidates allergen sensitization profiles in allergy to furry animals"],"prefix":"10.1111","volume":"51","author":[{"given":"Kyra","family":"Swiontek","sequence":"first","affiliation":[{"name":"Department of Infection and Immunity Luxembourg Institute of Health  Esch\u2010sur\u2010Alzette Luxembourg"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Kler","sequence":"additional","affiliation":[{"name":"Department of Infection and Immunity Luxembourg Institute of Health  Esch\u2010sur\u2010Alzette Luxembourg"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christiane","family":"Lehners","sequence":"additional","affiliation":[{"name":"National Unit of Immunology\u2010Allergology, Centre Hospitalier  Luxembourg Luxembourg"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Ollert","sequence":"additional","affiliation":[{"name":"Department of Infection and Immunity Luxembourg Institute of Health  Esch\u2010sur\u2010Alzette Luxembourg"},{"name":"Department of Dermatology and Allergy Center Odense Research Center for Anaphylaxis University of Southern Denmark  Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Hentges","sequence":"additional","affiliation":[{"name":"National Unit of Immunology\u2010Allergology, Centre Hospitalier  Luxembourg Luxembourg"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9455-5442","authenticated-orcid":false,"given":"Christiane","family":"Hilger","sequence":"additional","affiliation":[{"name":"Department of Infection and Immunity Luxembourg Institute of Health  Esch\u2010sur\u2010Alzette Luxembourg"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,4,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2014.08.026"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2009.02093.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/cea.12050"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2011.02675.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.09.019"},{"key":"e_1_2_9_7_1","first-page":"41","article-title":"Allergy to laboratory animals: an epidemiological study","volume":"49","author":"Aoyama K","year":"1992","journal-title":"Br J Ind Med"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/pai.12563"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00076"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.09.052"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.10.017"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2012.02826.x"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(95)70233-4"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.5414\/ALX01842"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2011.03726.x"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1159\/000371420"},{"key":"e_1_2_9_17_1","unstructured":"OliverosJ.VENNY. 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