{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T11:10:16Z","timestamp":1783941016271,"version":"3.55.0"},"reference-count":63,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,12,28]],"date-time":"2021-12-28T00:00:00Z","timestamp":1640649600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Excessive production of IgE plays a major role in the pathology of food allergy. In an attempt to identify anti\u2010IgE natural products, <jats:italic>Arctium Lappa<\/jats:italic> was one of the most effective herbs among approximately 300\u00a0screened medicinal herbs. However, little is known about its anti\u2010IgE compounds.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To identify compounds from <jats:italic>Arctium Lappa<\/jats:italic> for targeted therapy on IgE production and explore their underlying mechanisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Liquid\u2010liquid extraction and column chromatographic methods were used to purify the compounds. IgE inhibitory effects were determined on IgE\u2010producing human myeloma U266 cells, peanut\u2010allergic murine model and PBMCs from food\u2010allergic patients. Genes involved in IgE inhibition in PBMCs were studied by RNA sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The main compounds isolated were identified as arctiin and arctigenin. Both compounds significantly inhibited IgE production in U266 cells, with arctigenin the most potent (IC50=5.09\u03bcg\/mL). Arctigenin (at a dose of 13\u00a0mg\/kg) markedly reduced peanut\u2010specific IgE levels, blocked hypothermia and histamine release in a peanut\u2010allergic mouse model. Arctigenin also significantly reduced IgE production and Th2 cytokines (IL\u20105, IL\u201013) by PBMCs. We found 479 differentially expressed genes in PBMCs with arctigenin treatment (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.001 and fold\u2011change \u22651.5), involving 24\u00a0gene ontology terms (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.001, FDR &lt;0.05); cell division was the most significant. Eleven genes including <jats:italic>UBE2C and BCL6<\/jats:italic> were validated by qPCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Arctigenin markedly inhibited IgE production in U266 cells, peanut\u2010allergic murine model and PBMCs from allergic patients by down\u2010regulating cell division, cell cycle\u2010related genes and up\u2010regulating anti\u2010inflammatory factors.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.14048","type":"journal-article","created":{"date-parts":[[2021,11,10]],"date-time":"2021-11-10T19:22:23Z","timestamp":1636572143000},"page":"250-264","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Anti\u2010IgE effect of small\u2010molecule\u2010compound arctigenin on food allergy in association with a distinct transcriptome profile"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4781-6299","authenticated-orcid":false,"given":"Mingzhuo","family":"Cao","sequence":"first","affiliation":[{"name":"Academy of Traditional Chinese Medicine Science Henan University of Chinese Medicine  Zhengzhou Henan 450000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changda","family":"Liu","sequence":"additional","affiliation":[{"name":"Academy of Traditional Chinese Medicine Science Henan University of Chinese Medicine  Zhengzhou Henan 450000 China"},{"name":"Department of Pediatrics Icahn School of Medicine at Mount Sinai  New York 10029 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6581-1515","authenticated-orcid":false,"given":"Kamal. 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