{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:24:01Z","timestamp":1791980641941,"version":"4.4.0"},"reference-count":23,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,2,8]],"date-time":"2022-02-08T00:00:00Z","timestamp":1644278400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Silkworm pupa (SWP) food anaphylaxis has been described frequently in Asian countries. However, false\u2010positive reactions by skin pricks and serum IgE (sIgE) tests to the extract complicate diagnosis, requiring identification of clinically relevant major allergens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>In this study, we characterized a novel SWP allergen, Bomb m 4, a 30\u2010kDa lipoprotein, and evaluated its diagnostic sensitivity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Bomb m 4 was identified by a proteomic analysis. This recombinant (r)Bomb m 4 was overexpressed in <jats:italic>Escherichia coli<\/jats:italic>, and the IgE reactivity by ELISA was compared with other reported allergenic proteins: Bomb m 1 (arginine kinase), 27\u2010kDa glycoprotein, Bomb m 3 (tropomyosin) using the serum samples from 17 SWP allergic patients and 11 asymptomatic sensitized subjects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>rBomb m 4\u2010specific IgE was recognized by all 17 SWP allergic patients. The 27\u2010kDa glycoprotein and Bomb m 1\u00a0sIgE were found in 35.3% and 0%, respectively, in the SWP allergic patients. ELISA sIgE reactivity increased significantly, when 4\u00a0M urea was added in serum samples. However, only 16% inhibition of sIgE reactivity to the whole SWP extract was exhibited by rBomb m 4, whereas more than 93% of self\u2010inhibition of rBomb m 4\u00a0sIgE was obtained, possibly due to the low abundance of Bomb m 4 in the extract. Three linear epitopes (81\u201395, 191\u2013205 and 224\u2013238 residues) of rBomb m 4 were identified. These epitopes are shown to be released by pepsin digestion. Receiver operator characteristic (ROC) analysis showed the highest diagnostic value of Bomb m 4 followed by Bomb m 1, 27\u2010kDa glycoprotein and Bomb m 3.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Bomb m 4 is the major allergen of SWP allergic patients. It has cryptic epitopes which are exposed to IgE antibodies with digestive enzymes. This recombinant Bomb m 4 allergen permits exact diagnosis of SWP allergy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.14095","type":"journal-article","created":{"date-parts":[[2022,1,14]],"date-time":"2022-01-14T07:32:20Z","timestamp":1642145540000},"page":"888-897","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Allergenic characterization of Bomb m 4, a 30\u2010kDa <i>Bombyx mori<\/i> lipoprotein 6 from silkworm pupa"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9887-1426","authenticated-orcid":false,"given":"Kyoung Yong","family":"Jeong","sequence":"first","affiliation":[{"name":"Department of Internal Medicine Institute of Allergy Yonsei University College of Medicine  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong Sun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Institute of Allergy Yonsei University College of Medicine  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji Eun","family":"Yuk","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Institute of Allergy Yonsei University College of Medicine  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hangyeol","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Institute of Allergy Yonsei University College of Medicine  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hye Jung","family":"Lee","sequence":"additional","affiliation":[{"name":"ProteomeTech Inc.  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuan Jung","family":"Kim","sequence":"additional","affiliation":[{"name":"ProteomeTech Inc.  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bum Joon","family":"Kim","sequence":"additional","affiliation":[{"name":"ProteomeTech Inc.  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kook\u2010Jin","family":"Lim","sequence":"additional","affiliation":[{"name":"ProteomeTech Inc.  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3605-5364","authenticated-orcid":false,"given":"Kyung Hee","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Institute of Allergy Yonsei University College of Medicine  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae\u2010Hyun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Institute of Allergy Yonsei University College of Medicine  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0249-8749","authenticated-orcid":false,"given":"Jung\u2010Won","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Institute of Allergy Yonsei University College of Medicine  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,2,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.12449"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2008.01838.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/10903127.2016.1204035"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389203720666190715091951"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2009.02300.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/falgy.2021.652275"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2016.31.8.1197"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.4168\/aair.2019.11.5.723"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1159\/000210375"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2016.31.1.18"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2017.6373"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000236761"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0108761"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg520"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9888(199902)34:2<105::AID-JMS768>3.0.CO;2-4"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/ACI.0000000000000441"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-7917.2007.00121.x"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10142-012-0281-4"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/jisesa\/iev057"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/1348-0421.12271"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2020.128461"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00122"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2013.06.052"}],"container-title":["Clinical &amp; Experimental Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cea.14095","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cea.14095","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cea.14095","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T06:59:07Z","timestamp":1692946747000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cea.14095"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,8]]},"references-count":23,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2022,7]]}},"alternative-id":["10.1111\/cea.14095"],"URL":"https:\/\/doi.org\/10.1111\/cea.14095","archive":["Portico"],"relation":{},"ISSN":["0954-7894","1365-2222"],"issn-type":[{"value":"0954-7894","type":"print"},{"value":"1365-2222","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,8]]},"assertion":[{"value":"2021-08-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-12-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}