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Patients in cluster 1\u00a0had higher frequency of blood eosinophils at baseline, were sensitized to less allergens and had better steroid responsiveness, measured as the pronounced leukocyte redistribution after the administration of systemic CS. Patients in cluster 2 were determined by the higher frequency of B\u2010cells and stronger IgE sensitization status to the multiple allergens. They also displayed higher steroid resistance, as the clinical correlate for the lower leukocyte redistribution after administration of systemic CS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The flow cytometry\u2010based profiling of the basic populations of immune cells in the blood and its analysis before and after systemic corticosteroid administration could improve personalized treatment approaches in patients with severe asthma.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.14128","type":"journal-article","created":{"date-parts":[[2022,3,19]],"date-time":"2022-03-19T09:30:12Z","timestamp":1647682212000},"page":"1183-1194","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Leukocyte redistribution as immunological biomarker of corticosteroid resistance in severe 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