{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:20:38Z","timestamp":1790706038127,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,4,7]],"date-time":"2022-04-07T00:00:00Z","timestamp":1649289600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002657","name":"Istanbul \u00dcniversitesi","doi-asserted-by":"publisher","award":["49482"],"award-info":[{"award-number":["49482"]}],"id":[{"id":"10.13039\/501100002657","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Obesity\u2010associated asthma (OA) is a difficult to treat asthma phenotype due to its severity and poor response to inhaled steroids. Early\u2010onset allergic (EoOA) and late\u2010onset non\u2010allergic (LoOA) OA are suggested subtypes of this phenotype. Natural Killer (NK) cells are key elements of innate immunity involved in cytotoxicity and immune regulation, with uncertain role in OA pathogenesis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Early\u2010onset allergic and LoOA patients together with obese non\u2010asthmatic (ONA) controls have been enrolled in the study. Peripheral blood samples have been collected for analysis. Percentages of total NK cells, CD3<jats:sup>\u2212<\/jats:sup>CD56<jats:sup>dim<\/jats:sup> and CD3<jats:sup>\u2212<\/jats:sup>CD56<jats:sup>bright<\/jats:sup> NK cell subsets, cytotoxic activity, intracellular interferon\u2010\u03b3, interleukin (IL)\u201010, IL\u201013, IL\u201017\u00a0secretion and activatory receptors (NKG2D, NKp46i and NKp44) have been investigated by flow cytometry. The effect of IL\u201012 and IL\u201023\u00a0stimulation on NK cells and intracellular cytokines in different groups have also been analysed and compared with unstimulated conditions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Results of ONA (<jats:italic>n<\/jats:italic>\u00a0=\u00a05, age 42\u00a0\u00b1\u00a08), EoOA (<jats:italic>n<\/jats:italic>\u00a0=\u00a05, age 42\u00a0\u00b1\u00a010) and LoOA (<jats:italic>n<\/jats:italic>\u00a0=\u00a08, age 46\u00a0\u00b1\u00a08) patients have analysed. Body Mass Index has been found to be negatively correlated with CD69\u00a0(<jats:italic>p\u00a0<\/jats:italic>=\u00a0.022, <jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.534). NKG2D receptor has been significantly low in CD56<jats:sup>dim<\/jats:sup> cells\u00a0of asthma population (<jats:italic>p\u00a0=<\/jats:italic>\u00a0.046). NKp44 receptor expression has increased after IL\u201012\u00a0stimulation in EoOA and control group (<jats:italic>p\u00a0=<\/jats:italic>\u00a0.02). Intracellular IL\u201010 content has increased in LoOA and control subjects (<jats:italic>p\u00a0<\/jats:italic>=\u00a0.018, <jats:italic>p\u00a0=<\/jats:italic>\u00a0.03) but not in the EoOA group. Intracellular IL\u201017\u00a0level has found be higher in allergic OA group. LoOA patients showed a decreased NK cytotoxicity compared with the early\u2010onset asthma group (<jats:italic>p\u00a0=<\/jats:italic>\u00a0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our study suggests an impaired NK receptor expression, activation and reduced cytotoxicity in OA patients together with variances between different subtypes of this phenotype. This data would be beneficial for tailoring a more personalized treatment strategy combatting steroid resistance and frequent exacerbations in this group of patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.14136","type":"journal-article","created":{"date-parts":[[2022,3,31]],"date-time":"2022-03-31T21:10:15Z","timestamp":1648761015000},"page":"1432-1439","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Functionality of natural killer cells in obese asthma phenotypes"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7113-9988","authenticated-orcid":false,"given":"Leyla","family":"Pur Ozyigit","sequence":"first","affiliation":[{"name":"Adult Allergy Service Glenfield Hospital University Hospitals of Leicester NHS Trust  Leicester UK"},{"name":"Department of Immunology Aziz Sancar Institute of Experimental Medicine Istanbul University  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esin Cetin","family":"Aktas","sequence":"additional","affiliation":[{"name":"Department of Immunology Aziz Sancar Institute of Experimental Medicine Istanbul University  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuf Metin","family":"Gelmez","sequence":"additional","affiliation":[{"name":"Department of Immunology Aziz Sancar Institute of Experimental Medicine Istanbul University  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayse Bilge","family":"Ozturk","sequence":"additional","affiliation":[{"name":"Department of Allergy and Immunology Faculty of Medicine Koc University  Istanbul"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bilun","family":"Gemicioglu","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Diseases Cerrahpasa Faculty of Medicine Istanbul University\u2010Cerrahpasa  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gunnur","family":"Deniz","sequence":"additional","affiliation":[{"name":"Department of Immunology Aziz Sancar Institute of Experimental Medicine Istanbul University  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,4,7]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/oby.2007.7"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rmed.2007.06.031"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.03.036"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/17476348.2018.1506331"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2016-209130"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2018.02.004"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.06.009"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1513\/AnnalsATS.201702-122AW"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200711-1754OC"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0036631"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.04.021"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/oby.20383"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0908481"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3365"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3423"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/cea.13265"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2013.08.004"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1183\/09059180.00012514"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12832"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1582"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-09-077438"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V97.10.3146"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1402529"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4906(01)02060-9"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1581"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07537"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1962"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.aam5446"},{"key":"e_1_2_10_30_1","unstructured":"Global Strategy for Asthma Management and Prevention.GINA asthma. 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