{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T19:02:21Z","timestamp":1790622141289,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,5,4]],"date-time":"2022-05-04T00:00:00Z","timestamp":1651622400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In mice models, eosinophils have been divided into different subpopulations with distinct phenotypes and functions, based on CD62L and CD101 patterns of membrane expression. Limited data are available in humans.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate eosinophils subpopulations in peripheral blood (PB) and nasal polyp tissue (NP) from severe eosinophilic asthma (SEA) patients plus concomitant chronic rhinosinusitis with nasal polyps (CRSwNP).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We recruited 23 SEA patients (14 with CRSwNP); as controls, we enrolled 15 non\u2010severe asthma patients, 15 allergic rhinitis patients without asthma and 15\u00a0healthy donors. Eosinophils were isolated from PB and NP and analysed by FACS. Eotaxin\u20103 and eotaxin\u20101\u00a0mRNA expression in NP tissue was also evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A significantly higher percentage of circulating CD62L<jats:sup>low<\/jats:sup> cells was observed in SEA, as compared with controls, expressing higher levels of CCR3, CD69 and lower levels of CD125 (IL\u20105R), CRTH2, CD86 and CD28 in comparison with CD62L<jats:sup>bright<\/jats:sup> cells. In NP, eosinophils showed a high proportion of CD62L<jats:sup>low<\/jats:sup> phenotype, significantly greater than that observed in PB. Surface expression of IL\u20103R, IL\u20105R, CD69 and CD86 was significantly higher in CD62L<jats:sup>low<\/jats:sup> eosinophils from NP than in those from blood. Moreover, eotaxin\u20103\u00a0mRNA expression positively correlated with the percentage of CD62L<jats:sup>low<\/jats:sup> cells in NP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Two different eosinophil subphenotypes can be identified in blood and NP of SEA patients, with a preferential accumulation of CD62L<jats:sup>low<\/jats:sup> inflammatory cells in NP.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cea.14153","type":"journal-article","created":{"date-parts":[[2022,5,1]],"date-time":"2022-05-01T16:03:35Z","timestamp":1651421015000},"page":"78-87","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["High proportion of inflammatory CD62L<sup>low<\/sup> eosinophils in blood and nasal polyps of severe asthma patients"],"prefix":"10.1111","volume":"53","author":[{"given":"Andrea","family":"Matucci","sequence":"first","affiliation":[{"name":"Immunoallergology Unit Careggi University Hospital  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